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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347103 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal CHD5 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal CHD5 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CHD5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB. WB performed by Sonia Mulero, Spanish National Cancer Research Centre (CNIO), Madrid , Spain .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human CHD5. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:93,500.
- Validation comment
- ELISA
