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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2777 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2777, RRID:AB_1577165
- Product name
- MAP3K12 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAP3K12.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references SP3 acts as a positive regulator on the core promoter of human ZPK gene.
MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase.
ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation.
Itoh A, Wang Z, Ito Y, Reddy UR, Itoh T
Biochemical and biophysical research communications 2004 Jan 16;313(3):612-8
Biochemical and biophysical research communications 2004 Jan 16;313(3):612-8
MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase.
Fukuyama K, Yoshida M, Yamashita A, Deyama T, Baba M, Suzuki A, Mohri H, Ikezawa Z, Nakajima H, Hirai S, Ohno S
The Journal of biological chemistry 2000 Jul 14;275(28):21247-54
The Journal of biological chemistry 2000 Jul 14;275(28):21247-54
ZPK inhibits PKA induced transcriptional activation by CREB and blocks retinoic acid induced neuronal differentiation.
Reddy UR, Basu A, Bannerman P, Ikegaki N, Reddy CD, Pleasure D
Oncogene 1999 Aug 5;18(31):4474-84
Oncogene 1999 Aug 5;18(31):4474-84
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAP3K12 polyclonal antibody (Cat # PAB2777) in HL-60 cell lysate (lane A) and in mouse kidney tissue lysate (lane B) . MAP3K12 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP3K12 polyclonal antibody (Cat # PAB2777) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
