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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-30697 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB33B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, MCF-7, MDA-MB-231, Mouse brain. Predicted reactivity: Mouse (96%), Rat (97%), Xenopus laevis (80%), Chicken (94%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB33B using 30 µg of A) U87-MG and B) MCF-7 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RAB33B polyclonal antibody (Product # PA5-30697) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAB33B was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAB33B Polyclonal Antibody (Product # PA5-30697) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAB33B Polyclonal Antibody (Product # PA5-30697). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. RAB33B Polyclonal Antibody (Product # PA5-30697) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAB33B was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAB33B Polyclonal Antibody (Product # PA5-30697) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), U-87 MG (Lane 3), NIH/3T3 (Lane 4), PC-12 (Lane 5), MDCK (Lane 6), COS7 (Lane 7) and tissue extract of Mouse Liver (Lane 8). The blot was probed with Anti-RAB33B Polyclonal Antibody (Product # PA5-30697, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 25 kDa band corresponding to RAB33B was observed across the cell lines and tissue tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using RAB33B (Product # PA5-30697) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAB33B Polyclonal Antibody detects RAB33B protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. RAB33B Polyclonal Antibody (Product # PA5-30697) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAB33B Polyclonal Antibody detects RAB33B protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. RAB33B Polyclonal Antibody (Product # PA5-30697) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
