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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN954036 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Protocadherin alpha 8 (PCDHa8) (C-Term), (AA 780-808) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region ( between 780-808 aa) of human PCDHA8.
- Description
- Purified through a protein A column; followed by peptide affinity purification.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 780-808
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) using PCDHA8 antibody Cat.-No. AP53202PU-N. This demonstrates the PCDHA8 antibody detected the PCDHA8 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ZR-75-1 cell using PCDHA8 antibody Cat.-No. AP53202PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human brain tissue using PCDHA8 antibody Cat.-No. AP53202PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHA8 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
