459120

antibody from Invitrogen Antibodies

Targeting: COX5A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

459120

Provider

Invitrogen Antibodies

Product name

COX5A Monoclonal Antibody (6E9B12D5)

Antibody type

Monoclonal

Antigen

Other

Description

Positive control: Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells.

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

6E9B12D5

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Ulcerative colitis mucosal transcriptomes reveal mitochondriopathy and personalized mechanisms underlying disease severity and treatment response.

Haberman Y, Karns R, Dexheimer PJ, Schirmer M, Somekh J, Jurickova I, Braun T, Novak E, Bauman L, Collins MH, Mo A, Rosen MJ, Bonkowski E, Gotman N, Marquis A, Nistel M, Rufo PA, Baker SS, Sauer CG, Markowitz J, Pfefferkorn MD, Rosh JR, Boyle BM, Mack DR, Baldassano RN, Shah S, Leleiko NS, Heyman MB, Grifiths AM, Patel AS, Noe JD, Aronow BJ, Kugathasan S, Walters TD, Gibson G, Thomas SD, Mollen K, Shen-Orr S, Huttenhower C, Xavier RJ, Hyams JS, Denson LA
Nature communications 2019 Jan 3;10(1):38

---

Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition.

Fan Y, Dickman KG, Zong WX
The Journal of biological chemistry 2010 Mar 5;285(10):7324-33

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Complex IV in isolated mitochondria using a Complex IV Monoclonal antibody (Product # 459120) at a concentration of 1 µg/mL. Lane 1: Human heart mitochondria at 5 µg, Lane 2: Bovine heart mitochondria at 1 µg, Lane 3: Rat heart mitochondria at 10 µg, Lane 4: Mouse heart mitochondria at 10 µg, Lane 5: HepG2 mitochondria at 20 µg. Predicted band size: 17 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Complex IV in MRC5 cells using a Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized, and then labeled with Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. An AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody at a concentration of 1 µg/mL was used for detection.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Complex IV in MRC5 cells using a Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized, and then labeled with Complex IV Monoclonal antibody (Product # 459120) at a concentration of 10 µg/mL. An AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody at a concentration of 1 µg/mL was used for detection.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Complex IV in HeLa cells using a Complex IV monoclonal antibody (Product # 459120) at 1µg/mL is depicted by the blue line. The red line indicates an isotype control antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Colonic mitochondriopathy with a robust gene signature for reduced rectal mitochondrial energy functions in UC. a Thirteen mitochondrial-encoded genes are downregulated in UC vs. control with their fold change, FDR corrected P value, and associated mitochondrial complex as indicated. High-Resolution Respirometry was performed on fresh colon biopsies (5 control, 9 with active UC, and 9 with inactive UC) using the Oroboros O2k modular system to evaluate the activity of Complex I ( b ) and Complex II ( c ) of the electron transport chain. JC1 staining and FACS analysis were used to define the mitochondrial membrane potential of d EpCAM + epithelial cells and e CD45 + leukocytes isolated from colon biopsies (7 controls, 6 active UC, and 7 with inactive UC, 85-99% viability). Colon PPARGC1A (PGC-1alpha) expression for f PROTECT cohort, h RISK cohort in (transcripts per million (TPM) values), and for j adult UC cohort (GSE59071 12 ) in normalized values was plotted after stratifying the samples as indicated. g , i , k Krebs cycle TCA gene signature PCA PC1 for the above cohorts is plotted, samples are stratified as indicated. l Representative rectal MT-CO1 and COX5A immunohistochemistry (complex IV) for Ctl ( n = 14), inactive ( n = 10), and active UC ( n = 11) with moderate Mayo endoscopic subscore and moderate PUCAI. Scale bar represents 50 mum. m Frequency of MT-CO1-positive and COX5A-positive epithelial cells out of the total epithelial cells for