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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P31B-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF beta Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.
- Concentration
- 0.1-1.0 mg/mL
Submitted references A novel small-molecule tumor necrosis factor α inhibitor attenuates inflammation in a hepatitis mouse model.
Ma L, Gong H, Zhu H, Ji Q, Su P, Liu P, Cao S, Yao J, Jiang L, Han M, Ma X, Xiong D, Luo HR, Wang F, Zhou J, Xu Y
The Journal of biological chemistry 2014 May 2;289(18):12457-66
The Journal of biological chemistry 2014 May 2;289(18):12457-66
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human TNF-beta by Western Blot analysis TNF beta Polyclonal Antibody (Product # 500-P31B-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TNF-beta is 1.5-3.0 ng/lane, under either reducing non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human TNF-beta by Western Blot analysis TNF beta Polyclonal Antibody (Product # 500-P31B-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TNF-beta is 1.5-3.0 ng/lane, under either reducing non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TNF beta Polyclonal Antibody (Product # 500-P31B-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TNF beta Polyclonal Antibody, Biotin (Product # 500-P31BBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TNF-beta.
