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- Antibody Data
- Antigen structure
- References [70]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF-410-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TNF-alpha in ELISAs. Detects human and mouse TNF-alpha in Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF-alpha is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P06804- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of tumour necrosis factor alpha in the R6/2 mouse model of Huntington's disease by etanercept treatment.
Neuropathology in intrauterine growth restricted newborn piglets is associated with glial activation and proinflammatory status in the brain.
Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice.
A Dual Noradrenergic Mechanism for the Relief of Neuropathic Allodynia by the Antidepressant Drugs Duloxetine and Amitriptyline.
AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner.
Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis.
The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia.
Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice.
Gender difference in NASH susceptibility: Roles of hepatocyte Ikkβ and Sult1e1.
Subversion of NK-cell and TNFα Immune Surveillance Drives Tumor Recurrence.
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-β and cognitive function in APP/PS1 mice.
A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity.
Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice.
Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia.
Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis.
Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-κB Activation.
Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells.
Pathophysiology of lung injury induced by common bile duct ligation in mice.
Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus.
Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
No effect of ablation of surfactant protein-D on acute cerebral infarction in mice.
FcγRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis.
Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis.
Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions.
Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Rod-Shaped monocytes patrol the brain vasculature and give rise to perivascular macrophages under the influence of proinflammatory cytokines and angiopoietin-2.
Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Antipsoriatic effects of avarol-3'-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin.
TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
T lineage differentiation from human embryonic stem cells.
The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation.
Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
Sequential ELISA to profile multiple cytokines from small volumes.
Selective macrophage suppression during sepsis.
Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha.
Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha.
Pido-Lopez J, Tanudjojo B, Farag S, Bondulich MK, Andre R, Tabrizi SJ, Bates GP
Scientific reports 2019 May 10;9(1):7202
Scientific reports 2019 May 10;9(1):7202
Neuropathology in intrauterine growth restricted newborn piglets is associated with glial activation and proinflammatory status in the brain.
Wixey JA, Lee KM, Miller SM, Goasdoue K, Colditz PB, Tracey Bjorkman S, Chand KK
Journal of neuroinflammation 2019 Jan 8;16(1):5
Journal of neuroinflammation 2019 Jan 8;16(1):5
Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice.
Righetti RF, Dos Santos TM, Camargo LDN, Aristóteles LRCRB, Fukuzaki S, de Souza FCR, Santana FPR, de Agrela MVR, Cruz MM, Alonso-Vale MIC, Genaro IS, Saraiva-Romanholo BM, Leick EA, Martins MA, Prado CM, Tibério IFLC
Frontiers in pharmacology 2018;9:1021
Frontiers in pharmacology 2018;9:1021
A Dual Noradrenergic Mechanism for the Relief of Neuropathic Allodynia by the Antidepressant Drugs Duloxetine and Amitriptyline.
Kremer M, Yalcin I, Goumon Y, Wurtz X, Nexon L, Daniel D, Megat S, Ceredig RA, Ernst C, Turecki G, Chavant V, Théroux JF, Lacaud A, Joganah LE, Lelievre V, Massotte D, Lutz PE, Gilsbach R, Salvat E, Barrot M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Nov 14;38(46):9934-9954
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Nov 14;38(46):9934-9954
AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner.
Zenke K, Muroi M, Tanamoto KI
Cellular signalling 2018 Mar;43:32-39
Cellular signalling 2018 Mar;43:32-39
Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis.
Lee CJ, Moon SJ, Jeong JH, Lee S, Lee MH, Yoo SM, Lee HS, Kang HC, Lee JY, Lee WS, Lee HJ, Kim EK, Jhun JY, Cho ML, Min JK, Cho YY
Cell death & disease 2018 Mar 14;9(3):401
Cell death & disease 2018 Mar 14;9(3):401
The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia.
Widmann C, Gandin C, Petit-Paitel A, Lazdunski M, Heurteaux C
Scientific reports 2018 Dec 24;8(1):18062
Scientific reports 2018 Dec 24;8(1):18062
Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice.
Oh Y, Kwon YS, Jung BD
International journal of medical sciences 2017;14(8):729-734
International journal of medical sciences 2017;14(8):729-734
Gender difference in NASH susceptibility: Roles of hepatocyte Ikkβ and Sult1e1.
Matsushita N, Hassanein MT, Martinez-Clemente M, Lazaro R, French SW, Xie W, Lai K, Karin M, Tsukamoto H
PloS one 2017;12(8):e0181052
PloS one 2017;12(8):e0181052
Subversion of NK-cell and TNFα Immune Surveillance Drives Tumor Recurrence.
Kottke T, Evgin L, Shim KG, Rommelfanger D, Boisgerault N, Zaidi S, Diaz RM, Thompson J, Ilett E, Coffey M, Selby P, Pandha H, Harrington K, Melcher A, Vile R
Cancer immunology research 2017 Nov;5(11):1029-1045
Cancer immunology research 2017 Nov;5(11):1029-1045
Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-β and cognitive function in APP/PS1 mice.
Dempsey C, Rubio Araiz A, Bryson KJ, Finucane O, Larkin C, Mills EL, Robertson AAB, Cooper MA, O'Neill LAJ, Lynch MA
Brain, behavior, and immunity 2017 Mar;61:306-316
Brain, behavior, and immunity 2017 Mar;61:306-316
A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity.
Chung KJ, Chatzigeorgiou A, Economopoulou M, Garcia-Martin R, Alexaki VI, Mitroulis I, Nati M, Gebler J, Ziemssen T, Goelz SE, Phieler J, Lim JH, Karalis KP, Papayannopoulou T, Blüher M, Hajishengallis G, Chavakis T
Nature immunology 2017 Jun;18(6):654-664
Nature immunology 2017 Jun;18(6):654-664
Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice.
Muñoz MC, Burghi V, Miquet JG, Cervino IA, Quiroga DT, Mazziotta L, Dominici FP
Peptides 2017 Feb;88:37-45
Peptides 2017 Feb;88:37-45
Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia.
Tsunekawa T, Banno R, Mizoguchi A, Sugiyama M, Tominaga T, Onoue T, Hagiwara D, Ito Y, Iwama S, Goto M, Suga H, Sugimura Y, Arima H
EBioMedicine 2017 Feb;16:172-183
EBioMedicine 2017 Feb;16:172-183
Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis.
Lee SH, Kwon JY, Kim SY, Jung K, Cho ML
Scientific reports 2017 Aug 31;7(1):10133
Scientific reports 2017 Aug 31;7(1):10133
Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-κB Activation.
Ha SK, Sung J, Choi I, Kim Y
Pharmacognosy magazine 2016 Oct-Dec;12(48):295-301
Pharmacognosy magazine 2016 Oct-Dec;12(48):295-301
Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells.
Lim SY, Yuzhalin AE, Gordon-Weeks AN, Muschel RJ
Oncogene 2016 Nov 3;35(44):5735-5745
Oncogene 2016 Nov 3;35(44):5735-5745
Pathophysiology of lung injury induced by common bile duct ligation in mice.
Shikata F, Sakaue T, Nakashiro K, Okazaki M, Kurata M, Okamura T, Okura M, Ryugo M, Nakamura Y, Yasugi T, Higashiyama S, Izutani H
PloS one 2014;9(4):e94550
PloS one 2014;9(4):e94550
Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus.
Ilett E, Kottke T, Donnelly O, Thompson J, Willmon C, Diaz R, Zaidi S, Coffey M, Selby P, Harrington K, Pandha H, Melcher A, Vile R
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Oct;22(10):1851-63
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Oct;22(10):1851-63
Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
Ingham V, Williams A, Bate C
Journal of neuroinflammation 2014 Jun 21;11:115
Journal of neuroinflammation 2014 Jun 21;11:115
No effect of ablation of surfactant protein-D on acute cerebral infarction in mice.
Lambertsen KL, Østergaard K, Clausen BH, Hansen S, Stenvang J, Thorsen SB, Meldgaard M, Kristensen BW, Hansen PB, Sorensen GL, Finsen B
Journal of neuroinflammation 2014 Jul 19;11:123
Journal of neuroinflammation 2014 Jul 19;11:123
FcγRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis.
Clatworthy MR, Harford SK, Mathews RJ, Smith KG
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):17971-6
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):17971-6
Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
Ramkhelawon B, Hennessy EJ, Ménager M, Ray TD, Sheedy FJ, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner KJ, Moore KJ
Nature medicine 2014 Apr;20(4):377-84
Nature medicine 2014 Apr;20(4):377-84
Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
Junkins RD, MacNeil AJ, Wu Z, McCormick C, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5178-86
Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
Li B, Shi Y, Shu J, Gao J, Wu P, Tang SJ
The Journal of biological chemistry 2013 May 10;288(19):13610-9
The Journal of biological chemistry 2013 May 10;288(19):13610-9
Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis.
Hillian AD, McMullen MR, Sebastian BM, Roychowdhury S, Kashyap SR, Schauer PR, Kirwan JP, Feldstein AE, Nagy LE
The Journal of biological chemistry 2013 Aug 2;288(31):22565-75
The Journal of biological chemistry 2013 Aug 2;288(31):22565-75
Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H
PloS one 2012;7(4):e35588
PloS one 2012;7(4):e35588
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
The Journal of clinical investigation 2011 Oct;121(10):3846-59
The Journal of clinical investigation 2011 Oct;121(10):3846-59
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Wendt W, Schulten R, Stichel CC, Lübbert H
Journal of neurochemistry 2009 Sep;110(6):1931-41
Journal of neurochemistry 2009 Sep;110(6):1931-41
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
The Journal of clinical investigation 2009 Oct;119(10):2954-64
The Journal of clinical investigation 2009 Oct;119(10):2954-64
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions.
Joly S, Francke M, Ulbricht E, Beck S, Seeliger M, Hirrlinger P, Hirrlinger J, Lang KS, Zinkernagel M, Odermatt B, Samardzija M, Reichenbach A, Grimm C, Remé CE
The American journal of pathology 2009 Jun;174(6):2310-23
The American journal of pathology 2009 Jun;174(6):2310-23
Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
Journal of neurochemistry 2009 Dec;111(6):1409-24
Journal of neurochemistry 2009 Dec;111(6):1409-24
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
Microbes and infection 2009 Apr;11(4):524-30
Microbes and infection 2009 Apr;11(4):524-30
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology 2008 Oct;125(2):272-9
Immunology 2008 Oct;125(2):272-9
Rod-Shaped monocytes patrol the brain vasculature and give rise to perivascular macrophages under the influence of proinflammatory cytokines and angiopoietin-2.
Audoy-Rémus J, Richard JF, Soulet D, Zhou H, Kubes P, Vallières L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 8;28(41):10187-99
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 8;28(41):10187-99
Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages.
Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H
American journal of physiology. Cell physiology 2008 Jul;295(1):C2-12
American journal of physiology. Cell physiology 2008 Jul;295(1):C2-12
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
Biochemical pharmacology 2008 Jul 1;76(1):79-90
Biochemical pharmacology 2008 Jul 1;76(1):79-90
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2553-61
Antipsoriatic effects of avarol-3'-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin.
Amigó M, Payá M, De Rosa S, Terencio MC
British journal of pharmacology 2007 Oct;152(3):353-65
British journal of pharmacology 2007 Oct;152(3):353-65
TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
Cunha TM, Verri WA Jr, Fukada SY, Guerrero AT, Santodomingo-Garzón T, Poole S, Parada CA, Ferreira SH, Cunha FQ
European journal of pharmacology 2007 Nov 14;573(1-3):221-9
European journal of pharmacology 2007 Nov 14;573(1-3):221-9
Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2006 Nov;55(11):457-64
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2006 Nov;55(11):457-64
Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
Journal of ethnopharmacology 2006 Jul 19;106(3):418-24
Journal of ethnopharmacology 2006 Jul 19;106(3):418-24
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
Molecular and cellular neurosciences 2006 Jan;31(1):149-60
Molecular and cellular neurosciences 2006 Jan;31(1):149-60
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjöstedt A, Edebro H, Forsman M, Byström M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infection and immunity 2006 Dec;74(12):6730-8
Infection and immunity 2006 Dec;74(12):6730-8
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 15;177(12):8650-7
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
European journal of immunology 2006 Aug;36(8):2025-34
European journal of immunology 2006 Aug;36(8):2025-34
Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
Murata T, Tsuboi M, Hikita K, Kaneda N
The Journal of biological chemistry 2006 Aug 11;281(32):22503-16
The Journal of biological chemistry 2006 Aug 11;281(32):22503-16
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 1;103(31):11784-9
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 1;103(31):11784-9
T lineage differentiation from human embryonic stem cells.
Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 1;103(31):11742-7
Proceedings of the National Academy of Sciences of the United States of America 2006 Aug 1;103(31):11742-7
The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS
The Journal of investigative dermatology 2005 Nov;125(5):1053-62
The Journal of investigative dermatology 2005 Nov;125(5):1053-62
Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation.
Polavarapu R, Gongora MC, Winkles JA, Yepes M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 Nov 2;25(44):10094-100
The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 Nov 2;25(44):10094-100
Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
Mbebi C, González de Aguilar JL, Sée V, Dupuis L, Frossard N, Mercken L, Pradier L, Larmet Y, Loeffler JP
Neurobiology of disease 2005 Jun-Jul;19(1-2):129-41
Neurobiology of disease 2005 Jun-Jul;19(1-2):129-41
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
Tezel G, Yang X, Yang J, Wax MB
Brain research 2004 Jan 23;996(2):202-12
Brain research 2004 Jan 23;996(2):202-12
Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
Tezel G, Yang X, Yang J, Wax MB
Brain research 2004 Jan 23;996(2):202-12
Brain research 2004 Jan 23;996(2):202-12
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Jeyaseelan S, Chu HW, Young SK, Worthen GS
Infection and immunity 2004 Dec;72(12):7247-56
Infection and immunity 2004 Dec;72(12):7247-56
Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
LaBue M, Colburn KK, Green LM
Thyroid : official journal of the American Thyroid Association 2004 Apr;14(4):249-62
Thyroid : official journal of the American Thyroid Association 2004 Apr;14(4):249-62
Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha.
De SK, Devadas K, Notkins AL
Journal of virology 2002 Nov;76(22):11710-4
Journal of virology 2002 Nov;76(22):11710-4
Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha.
De SK, Devadas K, Notkins AL
Journal of virology 2002 Nov;76(22):11710-4
Journal of virology 2002 Nov;76(22):11710-4
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Supportive validation
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- Main image
- Experimental details
- Detection of Mouse TNF-alpha by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TNF-alpha at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in RAW 264.7 Mouse Cell Line. TNF-alpha was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Human/Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
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- TNF-alpha in Mouse T Cells. TNF-alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Human/Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody. Recombinant Mouse TNF-alpha (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-alpha (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND50 is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.
