LS-C745236

antibody from LSBio

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

LS-C745236

Provider

LSBio

Product name

TNF Alpha Antibody LS-C745236

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry using the polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.