- PA5-46945 - Invitrogen Antibodies TNF antibody

Submitted references Seed-induced Aβ deposition alters neuronal function and impairs olfaction in a mouse model of Alzheimer's disease.

Ziegler-Waldkirch S, Friesen M, Loreth D, Sauer JF, Kemna S, Hilse A, Erny D, Helm C, D Errico P, Prinz M, Bartos M, Meyer-Luehmann M
Molecular psychiatry 2022 Oct;27(10):4274-4284

Reduction of the RNA Binding Protein TIA1 Exacerbates Neuroinflammation in Tauopathy.

LeBlang CJ, Medalla M, Nicoletti NW, Hays EC, Zhao J, Shattuck J, Cruz AL, Wolozin B, Luebke JI
Frontiers in neuroscience 2020;14:285

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TNF alpha in CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for TNF‚α at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TNF alpha in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TNF alpha in immersion fixed activated mouse T Cells. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 15 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TNF alpha in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TNF alpha in immersion fixed activated mouse T Cells. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 15 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of TNF alpha in immersion fixed paraffin-embedded sections of mouse spleen tissue. Samples were incubated with TNF alpha polyclonal antibody (Product # PA5-46945) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of mouse TNF-a is shown by increase in RFU (relative fluorescent units - measure of decrease in cytotoxicity) with increasing concentrations of TNF-a polyclonal antibody (PA5-46945).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Neutralization of TNF alpha in L‚929 mouse fibroblast cell line. Samples were incubated in TNF alpha polyclonal antibody (Product # PA5-46945). Recombinant Mouse TNF‚α induces cytotoxicity in the L‚929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‚α (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF‚α Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 Reduction of TIA1 increases production of TNFalpha in tauopathy. (A) log 2 fold change of TNFalpha expression in WT TIA1 +/+ and P301S subjects that are TIA1 +/+ , TIA1 +/- , and TIA1 -/- at 5 months (light gray) and 9 months (dark gray). n = 3 animals/genotype (B) Percent area occupied by TNFalpha particles in (C) . 5 months (light gray), 9 months (dark gray). n = 9-12 fields, from 3-4 animals/genotype. (C) High resolution (40x) confocal image stacks depicting the apex and adjacent dentate gyrus including the dorsal and ventral dentate granule cell layers and hilus in P301S subjects that are TIA1 +/+ , TIA1 +/- and TIA1 -/- at 5 months (top) and 9 months (bottom). TNFalpha visualized in purple. Scale = 25 mum. All statistical comparisons calculated with One-Way ANOVA with Bonferroni post hoc test, * p

PA5-46945

Antibody data