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- Product number
- GTX110520 - Provider product page
- Provider
- GeneTex
- Product name
- TNF alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Vildagliptin Reduces Stenosis of Injured Carotid Artery in Diabetic Mouse Through Inhibiting Vascular Smooth Muscle Cell Proliferation via ER Stress/NF-κB Pathway.
Fasudil, a Rho-Associated Coiled Coil-Forming Protein Kinase Inhibitor, Recovers Methylmercury-Induced Axonal Degeneration by Changing Microglial Phenotype in Rats.
Maternal E-Cigarette Exposure in Mice Alters DNA Methylation and Lung Cytokine Expression in Offspring.
Poly (ethylene-co-vinyl alcohol) is a suitable substrate for human olfactory neuroepithelial cell differentiation in vitro through a defined regulatory pathway.
Hyperglycemia activates the renin-angiotensin system and induces epithelial-mesenchymal transition in streptozotocin-induced diabetic kidneys.
Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro.
Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl-p53 pathway.
Topical insulin accelerates cutaneous wound healing in insulin-resistant diabetic rats.
Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase.
IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.
FTY720 Attenuates Angiotensin II-Induced Podocyte Damage via Inhibiting Inflammatory Cytokines.
Anti-inflammation performance of curcumin-loaded mesoporous calcium silicate cement.
Promotion of olfactory receptor neuron differentiation of olfactory neuroepithelial cells by using chitosan solution.
VHL Inactivation in Precancerous Kidney Cells Induces an Inflammatory Response via ER Stress-Activated IRE1α Signaling.
Galangin ameliorates cisplatin-induced nephrotoxicity by attenuating oxidative stress, inflammation and cell death in mice through inhibition of ERK and NF-kappaB signaling.
Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.
Human mesenchymal stem cells attenuate pulmonary hypertension induced by prenatal lipopolysaccharide treatment in rats.
Vorinostat Modulates the Imbalance of T Cell Subsets, Suppresses Macrophage Activity, and Ameliorates Experimental Autoimmune Uveoretinitis.
The expression of thymosin β4 in chronic hepatitis B combined nonalcoholic fatty liver disease.
Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Gender disparity in LDL-induced cardiovascular damage and the protective role of estrogens against electronegative LDL.
Ji Y, Ge Y, Xu X, Ye S, Fan Y, Zhang J, Mei L, Zhang X, Ying L, Yang T, Li C
Frontiers in pharmacology 2019;10:142
Frontiers in pharmacology 2019;10:142
Fasudil, a Rho-Associated Coiled Coil-Forming Protein Kinase Inhibitor, Recovers Methylmercury-Induced Axonal Degeneration by Changing Microglial Phenotype in Rats.
Fujimura M, Usuki F, Nakamura A
Toxicological sciences : an official journal of the Society of Toxicology 2019 Mar 1;168(1):126-136
Toxicological sciences : an official journal of the Society of Toxicology 2019 Mar 1;168(1):126-136
Maternal E-Cigarette Exposure in Mice Alters DNA Methylation and Lung Cytokine Expression in Offspring.
Chen H, Li G, Chan YL, Chapman DG, Sukjamnong S, Nguyen T, Annissa T, McGrath KC, Sharma P, Oliver BG
American journal of respiratory cell and molecular biology 2018 Mar;58(3):366-377
American journal of respiratory cell and molecular biology 2018 Mar;58(3):366-377
Poly (ethylene-co-vinyl alcohol) is a suitable substrate for human olfactory neuroepithelial cell differentiation in vitro through a defined regulatory pathway.
Li ST, Young TH, Huang TW
Acta biomaterialia 2018 Mar 1;68:204-213
Acta biomaterialia 2018 Mar 1;68:204-213
Hyperglycemia activates the renin-angiotensin system and induces epithelial-mesenchymal transition in streptozotocin-induced diabetic kidneys.
Chen CM, Juan SH, Chou HC
Journal of the renin-angiotensin-aldosterone system : JRAAS 2018 Jul-Sep;19(3):1470320318803009
Journal of the renin-angiotensin-aldosterone system : JRAAS 2018 Jul-Sep;19(3):1470320318803009
Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro.
Liu B, Ding F, Hu D, Zhou Y, Long C, Shen L, Zhang Y, Zhang D, Wei G
Stem cell research & therapy 2018 Jan 12;9(1):7
Stem cell research & therapy 2018 Jan 12;9(1):7
Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl-p53 pathway.
Zhou Y, Que KT, Zhang Z, Yi ZJ, Zhao PX, You Y, Gong JP, Liu ZJ
Cancer medicine 2018 Aug;7(8):4012-4022
Cancer medicine 2018 Aug;7(8):4012-4022
Topical insulin accelerates cutaneous wound healing in insulin-resistant diabetic rats.
Yu T, Gao M, Yang P, Pei Q, Liu D, Wang D, Zhang X, Liu Y
American journal of translational research 2017;9(10):4682-4693
American journal of translational research 2017;9(10):4682-4693
Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase.
Chiang MH, Liang CJ, Liu CW, Pan BJ, Chen WP, Yang YF, Lee IT, Tsai JS, Lee CW, Chen YL
Frontiers in pharmacology 2017;8:819
Frontiers in pharmacology 2017;8:819
IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.
Gao W, Li F, Zhou Z, Xu X, Wu Y, Zhou S, Yin D, Sun D, Xiong J, Jiang R, Zhang J
Frontiers in neurology 2017;8:281
Frontiers in neurology 2017;8:281
FTY720 Attenuates Angiotensin II-Induced Podocyte Damage via Inhibiting Inflammatory Cytokines.
Su K, Zeng P, Liang W, Luo Z, Wang Y, Lv X, Han Q, Yan M, Chen C
Mediators of inflammation 2017;2017:3701385
Mediators of inflammation 2017;2017:3701385
Anti-inflammation performance of curcumin-loaded mesoporous calcium silicate cement.
Chen YC, Shie MY, Wu YA, Lee KA, Wei LJ, Shen YF
Journal of the Formosan Medical Association = Taiwan yi zhi 2017 Sep;116(9):679-688
Journal of the Formosan Medical Association = Taiwan yi zhi 2017 Sep;116(9):679-688
Promotion of olfactory receptor neuron differentiation of olfactory neuroepithelial cells by using chitosan solution.
Li ST, Young TH, Lin CF, Huang TW
American journal of rhinology & allergy 2017 Sep 1;31(5):289-292
American journal of rhinology & allergy 2017 Sep 1;31(5):289-292
VHL Inactivation in Precancerous Kidney Cells Induces an Inflammatory Response via ER Stress-Activated IRE1α Signaling.
Kuo CY, Lin CH, Hsu T
Cancer research 2017 Jul 1;77(13):3406-3416
Cancer research 2017 Jul 1;77(13):3406-3416
Galangin ameliorates cisplatin-induced nephrotoxicity by attenuating oxidative stress, inflammation and cell death in mice through inhibition of ERK and NF-kappaB signaling.
Huang YC, Tsai MS, Hsieh PC, Shih JH, Wang TS, Wang YC, Lin TH, Wang SH
Toxicology and applied pharmacology 2017 Aug 15;329:128-139
Toxicology and applied pharmacology 2017 Aug 15;329:128-139
Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.
Liu Q, Long Z, Dong X, Zhang T, Zhao J, Sun B, Zhu J, Li J, Wang Q, Yang Z, Hu X, Li L
Experimental & molecular medicine 2017 Apr 21;49(4):e319
Experimental & molecular medicine 2017 Apr 21;49(4):e319
Human mesenchymal stem cells attenuate pulmonary hypertension induced by prenatal lipopolysaccharide treatment in rats.
Chou HC, Lin W, Chen CM
Clinical and experimental pharmacology & physiology 2016 Oct;43(10):906-14
Clinical and experimental pharmacology & physiology 2016 Oct;43(10):906-14
Vorinostat Modulates the Imbalance of T Cell Subsets, Suppresses Macrophage Activity, and Ameliorates Experimental Autoimmune Uveoretinitis.
Fang S, Meng X, Zhang Z, Wang Y, Liu Y, You C, Yan H
Neuromolecular medicine 2016 Mar;18(1):134-45
Neuromolecular medicine 2016 Mar;18(1):134-45
The expression of thymosin β4 in chronic hepatitis B combined nonalcoholic fatty liver disease.
Liang J, Cai W, Han T, Jing L, Ma Z, Gao Y
Medicine 2016 Dec;95(52):e5763
Medicine 2016 Dec;95(52):e5763
Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.
Chen WL, Sheu JR, Chen RJ, Hsiao SH, Hsiao CJ, Chou YC, Chung CL, Hsiao G
PloS one 2015;10(9):e0137979
PloS one 2015;10(9):e0137979
Gender disparity in LDL-induced cardiovascular damage and the protective role of estrogens against electronegative LDL.
Lee AS, Chen WY, Chan HC, Hsu JF, Shen MY, Chang CM, Bair H, Su MJ, Chang KC, Chen CH
Cardiovascular diabetology 2014 Mar 25;13:64
Cardiovascular diabetology 2014 Mar 25;13:64
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein by Western blot analysis.A. 30 µg Raji whole cell lysate/extract12 % SDS-PAGETNF alpha antibody (GTX110520) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extractB. 30 µg whole cell lysate/extract of human TNF-transfected 293T cells12 % SDS-PAGETNF alpha antibody (GTX110520) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein by western blot analysis.A. 30 ?g NCI-H929 whole cell lysate/extract12% SDS-PAGETNF alpha antibody (GTX110520) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein by western blot analysis. Un-treated (-) and treated (+, 1?g/ml LPS treatment for 4hr) Raw264.7 whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) Raw264.7 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) Raw264.7 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) Raw264.7 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. TNF alpha antibody (GTX110520) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma . TNF alpha antibody (GTX110520) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TNF alpha antibody (GTX110520) diluted at 1:400.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TNF alpha antibody (GTX110520) diluted at 1:400.
