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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NBP1-19532 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19532, RRID:AB_1643202
- Product name
- Rabbit Polyclonal TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Tumor necrosis factor alpha mediates neuromuscular synapse elimination.
Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARδ-AMPK-PGC-1α Pathway in Dyslipidemic Conditions.
5-HT1AR alleviates Aβ-induced cognitive decline and neuroinflammation through crosstalk with NF-κB pathway in mice.
Identification of hub genes and key pathways of dietary advanced glycation end products‑induced non‑alcoholic fatty liver disease by bioinformatics analysis and animal experiments.
Multitarget Profiling of a Strigolactone Analogue for Early Events of Alzheimer's Disease: In Vitro Therapeutic Activities against Neuroinflammation.
High hydrostatic pressure induces atrial electrical remodeling through upregulation of inflammatory cytokines.
Siglec1 enhances inflammation through miR-1260-dependent degradation of IκBα in COPD.
P16(INK4a) played a critical role in exacerbating acute tubular necrosis in acute kidney injury.
A specific small-molecule inhibitor of protein kinase CδI activity improves metabolic dysfunction in human adipocytes from obese individuals.
Sphingosine-1-phosphate receptor 3 in the medial prefrontal cortex promotes stress resilience by reducing inflammatory processes.
Cardioprotective effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in rats.
Role of TNF-α in Regulating the Exercise Pressor Reflex in Rats With Femoral Artery Occlusion.
Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpio L.).
Effects of pulsed electromagnetic field therapy at different frequencies and durations on rotator cuff tendon-to-bone healing in a rat model.
Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction.
Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in an in vivo model of head and neck cancer.
BATF regulates collagen-induced arthritis by regulating T helper cell differentiation.
Periprosthetic UHMWPE Wear Debris Induces Inflammation, Vascularization, and Innervation After Total Disc Replacement in the Lumbar Spine.
An M1-like Macrophage Polarization in Decidual Tissue during Spontaneous Preterm Labor That Is Attenuated by Rosiglitazone Treatment.
Effect of overuse-induced tendinopathy on tendon healing in a rat supraspinatus repair model.
Cryotherapy Reduces Inflammatory Response Without Altering Muscle Regeneration Process and Extracellular Matrix Remodeling of Rat Muscle.
Expression of COX-2, IL-1β, TNF-α and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp.
Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.
Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model.
Prevention of diabetic nephropathy by compound 21, selective agonist of angiotensin type 2 receptors, in Zucker diabetic fatty rats.
α-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signalling.
Scutellarin attenuates hypertension-induced expression of brain Toll-like receptor 4/nuclear factor kappa B.
Evaluation of Bronchiolar and Alveolar Cell Injuries Induced by Short- and Long-term Exposure to Sidestream Smoke.
Fu XQ, Peng J, Wang AH, Luo ZG
Cell discovery 2020;6:9
Cell discovery 2020;6:9
Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARδ-AMPK-PGC-1α Pathway in Dyslipidemic Conditions.
Han YM, Lee YJ, Jang YN, Kim HM, Seo HS, Jung TW, Jeong JH
BioMed research international 2020;2020:7806860
BioMed research international 2020;2020:7806860
5-HT1AR alleviates Aβ-induced cognitive decline and neuroinflammation through crosstalk with NF-κB pathway in mice.
Wang M, Zong HF, Chang KW, Han H, Yasir Rizvi M, Iffat Neha S, Li ZY, Yang WN, Qian YH
International immunopharmacology 2020 Mar 3;82:106354
International immunopharmacology 2020 Mar 3;82:106354
Identification of hub genes and key pathways of dietary advanced glycation end products‑induced non‑alcoholic fatty liver disease by bioinformatics analysis and animal experiments.
Wang J, Liu H, Xie G, Cai W, Xu J
Molecular medicine reports 2020 Feb;21(2):685-694
Molecular medicine reports 2020 Feb;21(2):685-694
Multitarget Profiling of a Strigolactone Analogue for Early Events of Alzheimer's Disease: In Vitro Therapeutic Activities against Neuroinflammation.
Kurt B, Ozleyen A, Antika G, Yilmaz YB, Tumer TB
ACS chemical neuroscience 2020 Feb 19;11(4):501-507
ACS chemical neuroscience 2020 Feb 19;11(4):501-507
High hydrostatic pressure induces atrial electrical remodeling through upregulation of inflammatory cytokines.
Li X, Xue YM, Guo HM, Deng CY, Peng DW, Yang H, Wei W, Liu Y, Liu FZ, Wang ZY, Zhang MZ, Rao F, Wu SL
Life sciences 2020 Feb 1;242:117209
Life sciences 2020 Feb 1;242:117209
Siglec1 enhances inflammation through miR-1260-dependent degradation of IκBα in COPD.
Li S, Jiang L, Yang Y, Cao J, Zhang Q, Zhang J, Wang R, Deng X, Li Y
Experimental and molecular pathology 2020 Apr;113:104398
Experimental and molecular pathology 2020 Apr;113:104398
P16(INK4a) played a critical role in exacerbating acute tubular necrosis in acute kidney injury.
Gu X, Peng CY, Lin SY, Qin ZY, Liang JL, Chen HJ, Hou CX, Wang R, Du YQ, Jin JL, Yang ZJ
American journal of translational research 2019;11(6):3850-3861
American journal of translational research 2019;11(6):3850-3861
A specific small-molecule inhibitor of protein kinase CδI activity improves metabolic dysfunction in human adipocytes from obese individuals.
Sparks R, Lui A, Bader D, Patel R, Murr M, Guida W, Fratti R, Patel NA
The Journal of biological chemistry 2019 Oct 11;294(41):14896-14910
The Journal of biological chemistry 2019 Oct 11;294(41):14896-14910
Sphingosine-1-phosphate receptor 3 in the medial prefrontal cortex promotes stress resilience by reducing inflammatory processes.
Corbett BF, Luz S, Arner J, Pearson-Leary J, Sengupta A, Taylor D, Gehrman P, Ross R, Bhatnagar S
Nature communications 2019 Jul 17;10(1):3146
Nature communications 2019 Jul 17;10(1):3146
Cardioprotective effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in rats.
Bıçakçı N, Karaboğa I, Dökmeci AH, Güzel S, Fidanol Erboğa Z
Biotechnic & histochemistry : official publication of the Biological Stain Commission 2019 Aug;94(6):442-448
Biotechnic & histochemistry : official publication of the Biological Stain Commission 2019 Aug;94(6):442-448
Role of TNF-α in Regulating the Exercise Pressor Reflex in Rats With Femoral Artery Occlusion.
Xing J, Lu J, Li J
Frontiers in physiology 2018;9:1461
Frontiers in physiology 2018;9:1461
Imidacloprid exposure cause the histopathological changes, activation of TNF-α, iNOS, 8-OHdG biomarkers, and alteration of caspase 3, iNOS, CYP1A, MT1 gene expression levels in common carp (Cyprinus carpio L.).
Özdemir S, Altun S, Arslan H
Toxicology reports 2018;5:125-133
Toxicology reports 2018;5:125-133
Effects of pulsed electromagnetic field therapy at different frequencies and durations on rotator cuff tendon-to-bone healing in a rat model.
Huegel J, Choi DS, Nuss CA, Minnig MCC, Tucker JJ, Kuntz AF, Waldorff EI, Zhang N, Ryaby JT, Soslowsky LJ
Journal of shoulder and elbow surgery 2018 Mar;27(3):553-560
Journal of shoulder and elbow surgery 2018 Mar;27(3):553-560
Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction.
Mohamed R, Coucha M, Elshaer SL, Artham S, Lemtalsi T, El-Remessy AB
Biochimica et biophysica acta. Molecular basis of disease 2018 Mar;1864(3):746-757
Biochimica et biophysica acta. Molecular basis of disease 2018 Mar;1864(3):746-757
Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in an in vivo model of head and neck cancer.
Hoshikawa H, Kamitori K, Indo K, Mori T, Kamata M, Takahashi T, Tokuda M
Oncology letters 2018 Mar;15(3):3422-3428
Oncology letters 2018 Mar;15(3):3422-3428
BATF regulates collagen-induced arthritis by regulating T helper cell differentiation.
Park SH, Rhee J, Kim SK, Kang JA, Kwak JS, Son YO, Choi WS, Park SG, Chun JS
Arthritis research & therapy 2018 Aug 2;20(1):161
Arthritis research & therapy 2018 Aug 2;20(1):161
Periprosthetic UHMWPE Wear Debris Induces Inflammation, Vascularization, and Innervation After Total Disc Replacement in the Lumbar Spine.
Veruva SY, Lanman TH, Isaza JE, Freeman TA, Kurtz SM, Steinbeck MJ
Clinical orthopaedics and related research 2017 May;475(5):1369-1381
Clinical orthopaedics and related research 2017 May;475(5):1369-1381
An M1-like Macrophage Polarization in Decidual Tissue during Spontaneous Preterm Labor That Is Attenuated by Rosiglitazone Treatment.
Xu Y, Romero R, Miller D, Kadam L, Mial TN, Plazyo O, Garcia-Flores V, Hassan SS, Xu Z, Tarca AL, Drewlo S, Gomez-Lopez N
Journal of immunology (Baltimore, Md. : 1950) 2016 Mar 15;196(6):2476-2491
Journal of immunology (Baltimore, Md. : 1950) 2016 Mar 15;196(6):2476-2491
Effect of overuse-induced tendinopathy on tendon healing in a rat supraspinatus repair model.
Tucker JJ, Riggin CN, Connizzo BK, Mauck RL, Steinberg DR, Kuntz AF, Soslowsky LJ, Bernstein J
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2016 Jan;34(1):161-6
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2016 Jan;34(1):161-6
Cryotherapy Reduces Inflammatory Response Without Altering Muscle Regeneration Process and Extracellular Matrix Remodeling of Rat Muscle.
Vieira Ramos G, Pinheiro CM, Messa SP, Delfino GB, Marqueti Rde C, Salvini Tde F, Durigan JL
Scientific reports 2016 Jan 4;6:18525
Scientific reports 2016 Jan 4;6:18525
Expression of COX-2, IL-1β, TNF-α and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp.
Szylberg Ł, Janiczek M, Popiel A, Marszałek A
Archives of medical science : AMS 2016 Feb 1;12(1):172-8
Archives of medical science : AMS 2016 Feb 1;12(1):172-8
Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.
Acosta SA, Tajiri N, Hoover J, Kaneko Y, Borlongan CV
Stroke 2015 Sep;46(9):2616-27
Stroke 2015 Sep;46(9):2616-27
Effect of isolated hyperglycemia on native mechanical and biologic shoulder joint properties in a rat model.
Thomas SJ, Sarver JJ, Yannascoli SM, Tucker JJ, Kelly JD 4th, Ahima RS, Barbe MF, Soslowsky LJ
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2014 Nov;32(11):1464-70
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2014 Nov;32(11):1464-70
Prevention of diabetic nephropathy by compound 21, selective agonist of angiotensin type 2 receptors, in Zucker diabetic fatty rats.
Castoldi G, di Gioia CR, Bombardi C, Maestroni S, Carletti R, Steckelings UM, Dahlöf B, Unger T, Zerbini G, Stella A
American journal of physiology. Renal physiology 2014 Nov 15;307(10):F1123-31
American journal of physiology. Renal physiology 2014 Nov 15;307(10):F1123-31
α-catenin acts as a tumour suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signalling.
Piao HL, Yuan Y, Wang M, Sun Y, Liang H, Ma L
Nature cell biology 2014 Mar;16(3):245-54
Nature cell biology 2014 Mar;16(3):245-54
Scutellarin attenuates hypertension-induced expression of brain Toll-like receptor 4/nuclear factor kappa B.
Chen X, Shi X, Zhang X, Lei H, Long S, Su H, Pei Z, Huang R
Mediators of inflammation 2013;2013:432623
Mediators of inflammation 2013;2013:432623
Evaluation of Bronchiolar and Alveolar Cell Injuries Induced by Short- and Long-term Exposure to Sidestream Smoke.
Kwon KY, Jung HR, Hwang I, Choi WI
Korean journal of pathology 2012 Apr;46(2):151-61
Korean journal of pathology 2012 Apr;46(2):151-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNF alpha in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. Western immunoblot analysis for TNF-alpha in the rat cortex and striatum. Treatment with scutellarin significantly reduced the expression of these inflammatory markers in a dose-dependent manner. *P < 0.001 versus sham group; #P < 0.05, P < 0.001 versus NS group; &P < 0.001 versus low-dose group. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/mi/2013/432623/), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected immunopositivity of this protein in the tested tissue. Staining was primarily observed in the epithelial cells while some staining was present in mucosa muscularis also.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected diffused immunostaining of this protein in the tested tissue. Staining was primarily observed in the epithelial cells and some cells showed membrane positivity also.
