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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX83996 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83996, RRID:AB_10729103
- Product name
- NSE2 antibody [4H9]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSMCE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSMCE2.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-NSMCE2 mouse monoclonal antibody (GTX83996) immunofluorescent staining of COS7 cells transiently transfected with NSMCE2
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC207639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NSMCE2 antibody(GTX83996), and then analyzed by flow cytometry.
