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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA501632 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501632, RRID:AB_11126171
- Product name
- NSMCE2 (NSE2) mouse monoclonal antibody, clone OTI4A2 (formerly 4A2)
- Antibody type
- Monoclonal
- Description
- NSMCE2 (NSE2) mouse monoclonal antibody, clone OTI4A2 (formerly 4A2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NSMCE2
- Isotype
- IgG
- Antibody clone number
- OTI4A2
- Vial size
- 100 µl
- Concentration
- 0.49 mg/ml
Submitted references Rescue of collapsed replication forks is dependent on NSMCE2 to prevent mitotic DNA damage.
Pond KW, de Renty C, Yagle MK, Ellis NA
PLoS genetics 2019 Feb;15(2):e1007942
PLoS genetics 2019 Feb;15(2):e1007942
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSMCE2 (RC207639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSMCE2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-NSMCE2 mouse monoclonal antibody (TA501632) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NSMCE2(RC207639).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC207639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NSMCE2 antibody(TA501632), and then analyzed by flow cytometry.
- Validation comment
- FC
