sc-14021

antibody from Santa Cruz Biotechnology

Targeting: PTPN1 PTP1B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-14021

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-14021, RRID:AB_2174944

Product name

Anti-PTPN1

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Inhibition of PTP1B restores IRS1-mediated hepatic insulin signaling in IRS2-deficient mice.

González-Rodríguez A, Mas Gutierrez JA, Sanz-González S, Ros M, Burks DJ, Valverde AM
Diabetes 2010 Mar;59(3):588-99

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Silencing of SH-PTP2 defines a crucial role in the inactivation of epidermal growth factor receptor by 5-aminosalicylic acid in colon cancer cells

G Monteleone, L Franchi, D Fina, R Caruso, P Vavassori, I Monteleone, E Calabrese, G C Naccari, S Bellinvia, R Testi, F Pallone
Cell Death and Differentiation 2005 Aug;13(2):202-211

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 50

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.