A301-027A-T

antibody from Invitrogen Antibodies

Targeting: TBC1D9 GRAMD9, KIAA0882, MDR1

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-027A-T

Provider

Invitrogen Antibodies

Product name

TBC1D9/MDR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TBC1D9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D9 antibody (Product # A301-027A; lot A301-027A-1) used for IP at 6 µg per reaction. TBC1D9 was also immunoprecipitated by rabbit anti-TBC1D9 antibody (Product # A301-028A). For blotting immunoprecipitated TBC1D9, Product # A301-028A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TBC1D9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBC1D9 antibody A301-027A (lot A301-027A-1) used for IP at 6 µg per reaction. TBC1D9 was also immunoprecipitated by rabbit anti-TBC1D9 antibody A301-028A. For blotting immunoprecipitated TBC1D9, A301-028A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.