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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4302 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4302, RRID:AB_1575129
- Product name
- GCNT1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GCNT1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Genomic organization of core 2 and I branching beta-1,6-N-acetylglucosaminyltransferases. Implication for evolution of the beta-1,6-N-acetylglucosaminyltransferase gene family.
Expression cloning of a cDNA encoding UDP-GlcNAc:Gal beta 1-3-GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen.
Bierhuizen MF, Maemura K, Kudo S, Fukuda M
Glycobiology 1995 Jun;5(4):417-25
Glycobiology 1995 Jun;5(4):417-25
Expression cloning of a cDNA encoding UDP-GlcNAc:Gal beta 1-3-GalNAc-R (GlcNAc to GalNAc) beta 1-6GlcNAc transferase by gene transfer into CHO cells expressing polyoma large tumor antigen.
Bierhuizen MF, Fukuda M
Proceedings of the National Academy of Sciences of the United States of America 1992 Oct 1;89(19):9326-330
Proceedings of the National Academy of Sciences of the United States of America 1992 Oct 1;89(19):9326-330
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of GCNT1 polyclonal antibody (Cat # PAB4302) in CEM cell line lysates (35 ug/lane). GCNT1 (arrow) was detected using the purified GCNT1 polyclonal antibody (Cat # PAB4302).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCNT1 polyclonal antibody (Cat # PAB4302) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
