H00057484-M01

antibody from Abnova Corporation

Targeting: RNF150 KIAA1214

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00057484-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00057484-M01, RRID:AB_490047

Product name

RNF150 monoclonal antibody (M01), clone 5D11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RNF150.

Antigen sequence

LHTEKTECGRYGEHSPKQDARGEVVMASSAHDRLA
CDPNTKFAAPTRGKNWIALIPKGNCTYRDKIRNAF
LQNASAVVIFNVGSNTNETITMPHAGVEDIVAIMI
PEPKG


Isotype

IgG

Antibody clone number

5D11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RNF150 expression in transfected 293T cell line by RNF150 monoclonal antibody (M01), clone 5D11.Lane 1: RNF150 transfected lysate(34.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNF150 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol