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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-96983 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HS1BP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MQSPAVLVTS RRLQNAHTGL DLTVPQHQEV RGKMMSGHVE YQILVVTRLA AFKSAKHRPE DVVQFLVSKK YSEIEEFYQK LSSRYAAASL PPLPRKVLFV GESDIRERRA VFNEILRCVS KDAELAGSPE LLEFLGTRSP GAAGLTSRDS SVLDGTDSQT GNDEEAFDFF EEQDQVAEEG PPVQSLKGED AEESLEEEEA; Positive Samples: HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.75 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using HS1BP3 Polyclonal antibody (Product # PA5-96983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 15s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HS1BP3 in extracts of various cell lines using HS1BP3 Polyclonal Antibody (Product # PA5-96983) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HS1BP3 was performed in U2OS cells using HS1BP3 Polyclonal Antibody (Product # PA5-96983) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HS1BP3 in paraffin-embedded human stomach using HS1BP3 Polyclonal Antibody (Product # PA5-96983) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HS1BP3 in paraffin-embedded rat kidney using HS1BP3 Polyclonal Antibody (Product # PA5-96983) at a dilution of 1:100.
