Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-1303-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OSMR Monoclonal Antibody (AN-V2), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This monoclonal AN-V2 antibody reacts with the human oncostatin M (OSM) receptor. OSM, which belongs to the interleukin-6 cytokine family, is produced by T cells, activated monocytes, and dendritic cells. In humans, OSM can bind two unique receptor complexes. The type I OSM receptor is composed of the LIFR associated with gp130, and recognizes both LIF and OSM. The type II OSM receptor, which consists of gp130 and the OSMR subunit, specifically binds the cytokine, leading to activation of the JAK/STAT signaling pathway. Reports have also indicated a soluble form of the OSM receptor, which this antibody also recognizes. The OSM receptor is expressed in the bone marrow, endothelium, liver, lung, and numerous tumor cell lines. The AN-V2 monoclonal antibody recognizes the type II (beta) OSM receptor. Applications Reported: This AN-V2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AN-V2 antibody has been pre-titrated and tested by flow cytometric analysis of the A375 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AN-V2
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Functional proteogenomics reveals biomarkers and therapeutic targets in lymphomas.
Molecular and functional characterization of a soluble form of oncostatin M/interleukin-31 shared receptor.
Oncostatin M: signal transduction and biological activity.
Rolland DCM, Basrur V, Jeon YK, McNeil-Schwalm C, Fermin D, Conlon KP, Zhou Y, Ng SY, Tsou CC, Brown NA, Thomas DG, Bailey NG, Omenn GS, Nesvizhskii AI, Root DE, Weinstock DM, Faryabi RB, Lim MS, Elenitoba-Johnson KSJ
Proceedings of the National Academy of Sciences of the United States of America 2017 Jun 20;114(25):6581-6586
Proceedings of the National Academy of Sciences of the United States of America 2017 Jun 20;114(25):6581-6586
Molecular and functional characterization of a soluble form of oncostatin M/interleukin-31 shared receptor.
Diveu C, Venereau E, Froger J, Ravon E, Grimaud L, Rousseau F, Chevalier S, Gascan H
The Journal of biological chemistry 2006 Dec 1;281(48):36673-82
The Journal of biological chemistry 2006 Dec 1;281(48):36673-82
Oncostatin M: signal transduction and biological activity.
Gómez-Lechón MJ
Life sciences 1999;65(20):2019-30
Life sciences 1999;65(20):2019-30
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of Jurkat (left) and A375 (right) cell lines with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human Oncostatin M Receptor APC (purple histogram). Total viable cells were used for analysis.