Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4389 - Provider product page
- Provider
- R&D Systems
- Product name
- Human OSMR beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human OSM R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse OSM R beta is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q99650
- Isotype
- IgG
- Antibody clone number
- 469221
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human OSM R beta Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and OSM R beta knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human OSM R beta Monoclonal Antibody (Catalog # MAB4389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for OSM R beta at approximately 45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- OSM R beta Specificity is Shown by Flow Cytometry in Knockout Cell Line. OSM R beta knockout HeLa human cervical carcinoma cell line was stained with Mouse Anti-Human OSM R beta Monoclonal Antibody (Catalog # MAB4389, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). No staining in the OSM R beta knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.