MAB1224-100

antibody from R&D Systems

Targeting: PDCD1LG2 B7-DC, bA574F11.2, Btdc, CD273, PD-L2, PDL2

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1224-100

Provider

R&D Systems

Product name

Human PD-L2/B7-DC Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse PD-L2, recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, or rhB7-H3 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q9BQ51


Isotype

IgG

Antibody clone number

176611

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Cancer-associated fibroblasts induce antigen-specific deletion of CD8 ⁺ T Cells to protect tumour cells.

Lakins MA, Ghorani E, Munir H, Martins CP, Shields JD
Nature communications 2018 Mar 5;9(1):948

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PD-L1 expression and presence of TILs in small intestinal neuroendocrine tumours.

Lamarca A, Nonaka D, Breitwieser W, Ashton G, Barriuso J, McNamara MG, Moghadam S, Rogan J, Mansoor W, Hubner RA, Clark C, Chakrabarty B, Valle JW
Oncotarget 2018 Mar 13;9(19):14922-14938

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Classification of gallbladder cancer by assessment of CD8⁺ TIL and PD-L1 expression.

Lin J, Long J, Wan X, Chen J, Bai Y, Wang A, Yang X, Wu Y, Robson SC, Sang X, Zhao H
BMC cancer 2018 Jul 28;18(1):766

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Clinicopathologic analysis of programmed cell death-1 and programmed cell death-ligand 1 and 2 expressions in pulmonary adenocarcinoma: comparison with histology and driver oncogenic alteration status.

Koh J, Go H, Keam B, Kim MY, Nam SJ, Kim TM, Lee SH, Min HS, Kim YT, Kim DW, Jeon YK, Chung DH
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2015 Sep;28(9):1154-66

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IFN-gamma generates maturation-arrested dendritic cells that induce T cell hyporesponsiveness independent of Foxp3+ T-regulatory cell generation.

Rojas D, Krishnan R
Immunology letters 2010 Aug 16;132(1-2):31-7

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

PD-L2/B7-DC in Human Lung Cancer Tissue. PD-L2/B7-DC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB1224) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of PD-L2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Human PD-L2 Monoclonal Antibody (Catalog # MAB1224, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂ Secondary Antibody (Catalog # F0102B).