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LearnPA5-112438
antibody from Invitrogen Antibodies
Targeting: H2AZ2
H2AFV, H2AV, MGC10170, MGC10831, MGC1947
Western blot
ELISA Immunocytochemistry
Immunoprecipitation Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-112438 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H2A.ZK7ac Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.24 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Methyl Histone 1F0 (Lys101) in HepG2 whole cell lysate using a Methyl Histone 1F0 (Lys101) polyclonal antibody (Product # PA5-112436) at a dilution of 1:50. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Acetyl-H2A.ZK7ac (Lys7) in Hela cells using a Polyclonal antibody (Product # PA5-112438) at a dilution of 1:50. Hela cells (treated with 30mM sodium butyrate for 4 hour) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Acetyl-H2A.ZK7ac (Lys7) in Hela cells using a Acetyl-H2A.ZK7ac (Lys7) polyclonal antibody (Product # PA5-112438) at a dilution of 1:25. The cells were treated with 30mM sodium butyrate for 4 hours and counter stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation Assay of Acetyl-H2A.ZK7ac (Lys7) in Hela cells using a Acetyl-H2A.ZK7ac (Lys7) polyclonal antibody (Product # PA5-112438). 4 x 10^6 Hela cells were treated with 30 mM sodium butyrate for 4h, treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5 µg of the primary anibody or a control of normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Acetyl-H2A.ZK7ac (Lys7) in HepG2 whole cell lysate using a Acetyl-H2A.ZK7ac (Lys7) polyclonal antibody (Product # PA5-112438).
