Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005097-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005097-M01, RRID:AB_2158703
- Product name
- Mouse Monoclonal Protocadherin-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PCDH1 - protocadherin 1 (cadherin-like 1)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Protocadherin-1 Localization and Cell-Adhesion Function in Airway Epithelial Cells in Asthma.
Identification of PCDH1 as a novel susceptibility gene for bronchial hyperresponsiveness.
Faura Tellez G, Willemse BW, Brouwer U, Nijboer-Brinksma S, Vandepoele K, Noordhoek JA, Heijink I, de Vries M, Smithers NP, Postma DS, Timens W, Wiffen L, van Roy F, Holloway JW, Lackie PM, Nawijn MC, Koppelman GH
PloS one 2016;11(10):e0163967
PloS one 2016;11(10):e0163967
Identification of PCDH1 as a novel susceptibility gene for bronchial hyperresponsiveness.
Koppelman GH, Meyers DA, Howard TD, Zheng SL, Hawkins GA, Ampleford EJ, Xu J, Koning H, Bruinenberg M, Nolte IM, van Diemen CC, Boezen HM, Timens W, Whittaker PA, Stine OC, Barton SJ, Holloway JW, Holgate ST, Graves PE, Martinez FD, van Oosterhout AJ, Bleecker ER, Postma DS
American journal of respiratory and critical care medicine 2009 Nov 15;180(10):929-35
American journal of respiratory and critical care medicine 2009 Nov 15;180(10):929-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protocadherin-1 Antibody (5D5) [H00005097-M01] - PCDH1 monoclonal antibody (M01), clone 5D5 Analysis of PCDH1 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Detection limit for recombinant GST tagged PCDH1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Analysis of monoclonal antibody to PCDH1 on A-431 cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Protocadherin-1 Antibody (5D5) [H00005097-M01] - Analysis of monoclonal antibody to PCDH1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.