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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ABIN954026 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Protocadherin 1 (PCDH1) (N-Term), (AA 186-214) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 186-214 amino acids from the N-terminal region of human PCDH
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 186-214
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Identification of PCDH1 as a novel susceptibility gene for bronchial hyperresponsiveness.
Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.
Koppelman GH, Meyers DA, Howard TD, Zheng SL, Hawkins GA, Ampleford EJ, Xu J, Koning H, Bruinenberg M, Nolte IM, van Diemen CC, Boezen HM, Timens W, Whittaker PA, Stine OC, Barton SJ, Holloway JW, Holgate ST, Graves PE, Martinez FD, van Oosterhout AJ, Bleecker ER, Postma DS
American journal of respiratory and critical care medicine 2009 Nov 15;180(10):929-35
American journal of respiratory and critical care medicine 2009 Nov 15;180(10):929-35
Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.
Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ
Nature biotechnology 2005 Jan;23(1):94-101
Nature biotechnology 2005 Jan;23(1):94-101
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- PCDH1 Antibody (N-term) (Cat. #AP53193PU-N) western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35μg/lane).This demonstrates the PCDH1 antibody detected the PCDH1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PCDH1 Antibody (N-term)(Cat#AP53193PU-N) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- PCDH1 Antibody (N-term) (Cat. #AP53193PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
