Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-62010 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLEKHG5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KGDRKSTGLK LSKKKARRRH TDDPSKECFT LKFDLNVDIE TEIVPAMKKK SLGEVLLPVF ERKGIALGKV DIYLDQSNTP LSL
- Concentration
- 0.1 mg/mL
Submitted references Identification and characterization of a new isoform of small GTPase RhoE.
Dai Y, Luo W, Yue X, Ma W, Wang J, Chang J
Communications biology 2020 Oct 15;3(1):572
Communications biology 2020 Oct 15;3(1):572
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLEKHG5 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a PLEKHG5 Polyclonal Antibody (Product # PA5-62010).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PLEKHG5 in human cell line U-2 OS using a PLEKHG5 Polyclonal Antibody (Product # PA5-62010) shows localization to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PLEKHG5 in human cerebral cortex tissue shows strong nuclear positivity in neuronal and glial cells. Samples were probed using a PLEKHG5 Polyclonal Antibody (Product # PA5-62010).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Functional similarity between RhoE and RhoEalpha. a Co-IP assay detected binding of ROCK1 to both RhoE and RhoEalpha in HEK293 cells. b Confocal images of NIH-3T3 cells. Cells were transfected with the indicated plasmids and fixed 24 h after transfection. Flag-tagged proteins were stained with anti-flag antibody (red). Cytoskeleton actins were stained by phalloidin (green). Cell nuclei was stained by DAPI (blue). Scale bar: 10 um. c Co-IP assay detected binding of both RhoE and RhoEalpha to Syx and p190RhoGAP. HEK293 cells were transfected with indicated plasmids. Twenty four hours after transfection, cell lysates were used for Co-IP assay with anti-flag antibody.