Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- NB100-93340 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93340, RRID:AB_1236806
- Product name
- Rabbit Polyclonal CEP78 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CEP78 Antibody [NB100-93340] - Detection of Human CEP78 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CEP78 antibody NB100-93340 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CEP78 Antibody [NB100-93340] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-CEP78 used at a dilution of 1:250.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CEP78 Antibody [NB100-93340] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-93340 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CEP78 was also immunoprecipitated by NB100-93341, which recognizes a downstream epitope
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CEP78 Antibody [NB100-93340] - Detection of human CEP78 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CEP78 antibody NB100-93340 (lot NB100-93340-2) used for IP at 6 ug/mg lysate. CEP78 was also immunoprecipitated by a previous lot of this antibody (lot NB100-93340-1) and rabbit anti-CEP78 antibody NB100-93341. For blotting immunoprecipitated CEP78, NB100-93340 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.