A305-547A

antibody from Invitrogen Antibodies

Targeting: NGLY1 FLJ11005, PNG-1, PNG1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A305-547A

Provider

Invitrogen Antibodies

Product name

NGLY1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NGLY1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NGLY1 antibody A305-547A (lot A305-547A-1) used for IP at 6 µg per reaction. NGLY1 was also immunoprecipitated by rabbit anti-NGLY1 antibody BL21982. For blotting immunoprecipitated NGLY1, A305-547A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human NGLY1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NGLY1 antibody A305-547A (lot A305-547A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.