10501-1-AP

antibody from Proteintech Group

Targeting: PSAT1 PSA

Provider product page for 10501-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [33]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 10501-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10501-1-AP, RRID:AB_2172597
Product name: PSAT1 antibody
Antibody type: Polyclonal
Description: KD/KO validated PSAT1 antibody (Cat. #10501-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PSAT1 protein structure - 10501-1-AP shown in red.

Submitted references Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.

Shen L, Hu P, Zhang Y, Ji Z, Shan X, Ni L, Ning N, Wang J, Tian H, Shui G, Yuan Y, Li G, Zheng H, Yang XP, Huang D, Feng X, Li MJ, Liu Z, Wang T, Yu Q
Cell metabolism 2021 May 4;33(5):971-987.e6

Serine Biosynthesis Is a Metabolic Vulnerability in IDH2-Driven Breast Cancer Progression.

Barnabas GD, Lee JS, Shami T, Harel M, Beck L, Selitrennik M, Jerby-Arnon L, Erez N, Ruppin E, Geiger T
Cancer research 2021 Mar 15;81(6):1443-1456

Extracellular serine and glycine are required for mouse and human skeletal muscle stem and progenitor cell function.

Gheller BJ, Blum JE, Lim EW, Handzlik MK, Hannah Fong EH, Ko AC, Khanna S, Gheller ME, Bender EL, Alexander MS, Stover PJ, Field MS, Cosgrove BD, Metallo CM, Thalacker-Mercer AE
Molecular metabolism 2021 Jan;43:101106

Serine synthesis influences tamoxifen response in ER+ human breast carcinoma.

Metcalf S, Petri BJ, Kruer T, Green B, Dougherty S, Wittliff JL, Klinge CM, Clem BF
Endocrine-related cancer 2021 Jan;28(1):27-37

Serine Metabolism Controls Dental Pulp Stem Cell Aging by Regulating the DNA Methylation of p16.

Yang RL, Huang HM, Han CS, Cui SJ, Zhou YK, Zhou YH
Journal of dental research 2021 Jan;100(1):90-97

Proteomic profile of human dental follicle stem cells and apical papilla stem cells.

Lei T, Zhang X, Chen P, Li Q, Du H
Journal of proteomics 2021 Jan 16;231:103928

LncRNA MEG8 promotes NSCLC progression by modulating the miR-15a-5p-miR-15b-5p/PSAT1 axis.

Guo K, Qi D, Huang B
Cancer cell international 2021 Feb 1;21(1):84

Nuclear Pyruvate Kinase M2 (PKM2) Contributes to Phosphoserine Aminotransferase 1 (PSAT1)-Mediated Cell Migration in EGFR-Activated Lung Cancer Cells.

Biyik-Sit R, Kruer T, Dougherty S, Bradley JA, Wilkey DW, Merchant ML, Trent JO, Clem BF
Cancers 2021 Aug 4;13(16)

Bone metastatic breast cancer cells display downregulation of PKC-ζ with enhanced glutamine metabolism.

Tandon M, Othman AH, Winogradzki M, Pratap J
Gene 2021 Apr 5;775:145419

PSAT1 Regulated Oxidation-Reduction Balance Affects the Growth and Prognosis of Epithelial Ovarian Cancer.

Zhang Y, Li J, Dong X, Meng D, Zhi X, Yuan L, Yao L
OncoTargets and therapy 2020;13:5443-5453

TGF-β Promotes Metabolic Reprogramming in Lung Fibroblasts via mTORC1-dependent ATF4 Activation.

O'Leary EM, Tian Y, Nigdelioglu R, Witt LJ, Cetin-Atalay R, Meliton AY, Woods PS, Kimmig LM, Sun KA, Gökalp GA, Mutlu GM, Hamanaka RB
American journal of respiratory cell and molecular biology 2020 Nov;63(5):601-612

Overexpression of PSAT1 promotes metastasis of lung adenocarcinoma by suppressing the IRF1-IFNγ axis.

Chan YC, Chang YC, Chuang HH, Yang YC, Lin YF, Huang MS, Hsiao M, Yang CJ, Hua KT
Oncogene 2020 Mar;39(12):2509-2522

Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis.

Zhang J, Yang J, Yang N, Ma J, Lu D, Dong Y, Liang H, Liu D, Cang M
Lipids in health and disease 2020 Mar 13;19(1):39

Selective loss of phosphoserine aminotransferase 1 (PSAT1) suppresses migration, invasion, and experimental metastasis in triple negative breast cancer.

Metcalf S, Dougherty S, Kruer T, Hasan N, Biyik-Sit R, Reynolds L, Clem BF
Clinical & experimental metastasis 2020 Feb;37(1):187-197

The auxiliary diagnostic value of prostate-specific antigen and α-methylacyl-CoA racemase in prostate cancer.

Yang D, Shi X, Lei Y, Zhou X, Chen Q
Oncology letters 2020 Aug;20(2):1418-1422

SOX2 has dual functions as a regulator in the progression of neuroendocrine prostate cancer.

Li H, Wang L, Li Z, Geng X, Li M, Tang Q, Wu C, Lu Z
Laboratory investigation; a journal of technical methods and pathology 2020 Apr;100(4):570-582

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Sinha T, Naash MI, Al-Ubaidi MR
iScience 2020 Apr 24;23(4):101004

De novo synthesis of serine and glycine fuels purine nucleotide biosynthesis in human lung cancer tissues.

Fan TWM, Bruntz RC, Yang Y, Song H, Chernyavskaya Y, Deng P, Zhang Y, Shah PP, Beverly LJ, Qi Z, Mahan AL, Higashi RM, Dang CV, Lane AN
The Journal of biological chemistry 2019 Sep 6;294(36):13464-13477

Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.

Tian S, Feng J, Cao Y, Shen S, Cai Y, Yang D, Yan R, Wang L, Zhang H, Zhong X, Gao P
Life science alliance 2019 Oct;2(5)

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Bott AJ, Shen J, Tonelli C, Zhan L, Sivaram N, Jiang YP, Yu X, Bhatt V, Chiles E, Zhong H, Maimouni S, Dai W, Velasquez S, Pan JA, Muthalagu N, Morton J, Anthony TG, Feng H, Lamers WH, Murphy DJ, Guo JY, Jin J, Crawford HC, Zhang L, White E, Lin RZ, Su X, Tuveson DA, Zong WX
Cell reports 2019 Oct 29;29(5):1287-1298.e6

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC.

Zeng H, Castillo-Cabrera J, Manser M, Lu B, Yang Z, Strande V, Begue D, Zamponi R, Qiu S, Sigoillot F, Wang Q, Lindeman A, Reece-Hoyes JS, Russ C, Bonenfant D, Jiang X, Wang Y, Cong F
eLife 2019 Nov 19;8

The SIRT2/cMYC Pathway Inhibits Peroxidation-Related Apoptosis In Cholangiocarcinoma Through Metabolic Reprogramming.

Xu L, Wang L, Zhou L, Dorfman RG, Pan Y, Tang D, Wang Y, Yin Y, Jiang C, Zou X, Wu J, Zhang M
Neoplasia (New York, N.Y.) 2019 May;21(5):429-441

A novel inhibitor of tumorspheres reveals the activation of the serine biosynthetic pathway upon mitochondrial inhibition.

Subedi A, Muroi M, Futamura Y, Kawamura T, Aono H, Nishi M, Ryo A, Watanabe N, Osada H
FEBS letters 2019 Apr;593(8):763-776

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Wang YQ, Wang HL, Xu J, Tan J, Fu LN, Wang JL, Zou TH, Sun DF, Gao QY, Chen YX, Fang JY
Nature communications 2018 Feb 7;9(1):545

Serine alleviates oxidative stress via supporting glutathione synthesis and methionine cycle in mice.

Zhou X, He L, Wu C, Zhang Y, Wu X, Yin Y
Molecular nutrition & food research 2017 Nov;61(11)

Serine Is an Essential Metabolite for Effector T Cell Expansion.

Ma EH, Bantug G, Griss T, Condotta S, Johnson RM, Samborska B, Mainolfi N, Suri V, Guak H, Balmer ML, Verway MJ, Raissi TC, Tsui H, Boukhaled G, Henriques da Costa S, Frezza C, Krawczyk CM, Friedman A, Manfredi M, Richer MJ, Hess C, Jones RG
Cell metabolism 2017 Feb 7;25(2):345-357

A simple method based on Sanger sequencing and MS Word wildcard searching to identify Cas9-induced frameshift mutations.

Jie H, Li Z, Wang P, Zhao L, Zhang Q, Yao X, Song X, Zhao Y, Yao S
Laboratory investigation; a journal of technical methods and pathology 2017 Dec;97(12):1500-1507

Targeting glutamine metabolism and the focal adhesion kinase additively inhibits the mammalian target of the rapamycin pathway in spheroid cancer stem-like properties of ovarian clear cell carcinoma in vitro.

Sato M, Kawana K, Adachi K, Fujimoto A, Yoshida M, Nakamura H, Nishida H, Inoue T, Taguchi A, Ogishima J, Eguchi S, Yamashita A, Tomio K, Wada-Hiraike O, Oda K, Nagamatsu T, Osuga Y, Fujii T
International journal of oncology 2017 Apr;50(4):1431-1438

Overexpression of Phosphoserine Aminotransferase 1 (PSAT1) Predicts Poor Prognosis and Associates with Tumor Progression in Human Esophageal Squamous Cell Carcinoma.

Liu B, Jia Y, Cao Y, Wu S, Jiang H, Sun X, Ma J, Yin X, Mao A, Shang M
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;39(1):395-406

MicroRNA-340 Inhibits Esophageal Cancer Cell Growth and Invasion by Targeting Phosphoserine Aminotransferase 1.

Yan S, Jiang H, Fang S, Yin F, Wang Z, Jia Y, Sun X, Wu S, Jiang T, Mao A
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2015;37(1):375-86

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

Proteomic characterization of ovarian cancers identifying annexin-A4, phosphoserine aminotransferase, cellular retinoic acid-binding protein 2, and serpin B5 as histology-specific biomarkers.

Toyama A, Suzuki A, Shimada T, Aoki C, Aoki Y, Umino Y, Nakamura Y, Aoki D, Sato TA
Cancer science 2012 Apr;103(4):747-55

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10501-1-AP( PSAT1 Antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The PSAT1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human PSAT1. This antibody recognizes human antigen. The PSAT1 antibody has been validated for the following applications: ELISA, IHC, WB analysis.
Appendix: 10501-1-AP.pdf

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