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- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [8]
- Other assay [1]
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- Product number
- MA5-31434 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCL11B Monoclonal Antibody (CL6426)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL6426
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references B-cell lymphoma/leukaemia 11B (BCL11B) expression status helps distinguish early T-cell precursor acute lymphoblastic leukaemia/lymphoma (ETP-ALL/LBL) from other subtypes of T-cell ALL/LBL.
Abstracts from USCAP 2021: Hematopathology (664-756).
Laboratory investigation; a journal of technical methods and pathology 2021 Mar;101(Suppl 1):821-943
Human Adipose-derived mesenchymal stem cells promote lymphocyte apoptosis and alleviate atherosclerosis via miR-125b-1-3p/BCL11B signal axis.
Fang H, Wang W, El Hussein S, Morita K, Beird HC, Mitra A, Loghavi S, Lin P, Jabbour EJ, Khoury JD
British journal of haematology 2021 Sep;194(6):1034-1038
British journal of haematology 2021 Sep;194(6):1034-1038
Abstracts from USCAP 2021: Hematopathology (664-756).
Laboratory investigation; a journal of technical methods and pathology 2021 Mar;101(Suppl 1):821-943
Human Adipose-derived mesenchymal stem cells promote lymphocyte apoptosis and alleviate atherosclerosis via miR-125b-1-3p/BCL11B signal axis.
Yu C, Tang W, Lu R, Tao Y, Ren T, Gao Y
Annals of palliative medicine 2021 Feb;10(2):2123-2133
Annals of palliative medicine 2021 Feb;10(2):2123-2133
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL11B by a BCL11B monoclonal antibody (Product # MA5-31434). Analysis in human cerebral cortex tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL11B by a BCL11B monoclonal antibody (Product # MA5-31434). Analysis in mouse cerebral cortex tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in mouse hippocampus using a BCL11B monoclonal antibody (Product # MA5-31434). The analysis shows strong nuclear positivity in neurons in CA1 pyramidal layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in human tonsil and pancreas tissues using a BCL11B monoclonal antibody (Product # MA5-31434). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in human tonsil using a BCL11B monoclonal antibody (Product # MA5-31434). The analysis shows moderate to strong nuclear positivity, mainly in non - germinal center cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in human skin using a BCL11B monoclonal antibody (Product # MA5-31434). The analysis shows moderate to strong nuclear positivity in dermal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in human pancreas using a BCL11B monoclonal antibody (Product # MA5-31434). The analysis shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in mouse cerebral cortex using a BCL11B monoclonal antibody (Product # MA5-31434). The analysis shows moderate to strong nuclear positivity in neurons, mainly in layers 5-6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in mouse hippocampus using a BCL11B monoclonal antibody (Product # MA5-31434). The analysis shows strong nuclear positivity in neurons in CA1 pyramidal layer.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BCL11B in mouse hippocampus using a BCL11B monoclonal antibody (Product # MA5-31434). The analysis shows strong nuclear positivity in neurons in the granular cell layer of dentate gyrus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1 Fig BCL11B protein expression in T-ALL. A normal bone marrow control showed scattered reactive T cells with a heterogenous BCL11B staining pattern by immunohistochemistry (A, B). The haematoxylin and eosin staining of tissues and their corresponding BCL11B immunohistochemical stains in representative cases of T-ALL/LBL (C, D, ETP-ALL; E, F, thymic T-ALL/LBL). There are scattered small T cells in ETP-ALL cases in the background serving as internal positive controls (D). The percentage of cases with positive BCL11B expression was significantly lower in the patients with ETP-ALL compared to patients with other T-ALL/LBL subtypes (G), while no significant difference was found among the early, thymic and mature T-ALL/LBL groups (H). The median percentage of BCL11B-positive cells (at any scale) was lowest in ETP-ALL and higher in thymic and mature T-ALL/LBL (I, J). The H-score of BCL11B staining differed significantly between ETP-ALL and other T-ALL/LBL groups (K) and among the early, thymic and mature T-ALL/LBL (L). (T-)ALL, (T-cell) acute lymphoblastic leukaemia; BCL11B, B-cell lymphoma/leukaemia 11B; ETP, early T-cell precursor; LBL, lymphoblastic lymphoma.
