Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1-31264 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GRAF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX15916-PEP).. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of GRAF was performed by loading 35 µg (in RIPA buffer) of human heart lysates with (B) and without (A) blocking with the immunising peptide. Proteins were transferred to a membrane and probed with a GRAF Polyclonal Antibody (Product # PA1-31264) at a dilution of 0.3 µg/mL.