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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX116326 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116326, RRID:AB_10617287
- Product name
- SIN3B antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE GTX116326 diluted at 1:500
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SIN3B antibody [N1N2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term (GTX116326) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 £gg of SIN3B antibody [N1N2], N-term (GTX116326).Western blot analysis was performed using SIN3B antibody [N1N2], N-term (GTX116326).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using SIN3B(GTX116326) antibody at 1:100 dilution.
