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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX116417 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116417, RRID:AB_10616827
- Product name
- SIN3B antibody [N1N2-2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 5% SDS PAGE GTX116417 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE GTX116417 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SIN3B antibody [N1N2-2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SIN3B protein stained by SIN3B antibody [N1N2-2], N-term (GTX116417) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 £gg of SIN3B antibody [N1N2-2], N-term (GTX116417).Western blot analysis was performed using SIN3B antibody [N1N2-2], N-term (GTX116417).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
