FNab01770

antibody from FineTest Biotech

Targeting: CLP1 hClp1, HEAB

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Antibody Data

Product number

FNab01770

Provider

FineTest Biotech

Product name

CLP1 antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA(dsRNA), single-stranded RNA(ssRNA), double-stranded DNA(dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA(PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development(PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II(CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs(siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex(RISC). However, endogenous siRNAs and microRNAs(miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

CLP1, cleavage and polyadenylation
factor I subunit, homolog


Epitope

IgG

Isotype

IgG

Vial size

100¦Ìg

Concentration

Immunogen affinity purified

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)

Handling

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)