Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-119907 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL2RG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse spleen tissue lysates, SH-SY5Y, SiHa, human spleen tissue, human tonsil tissue.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of IL-2RG on mouse spleen tissue withIL2RG Polyclonal Antibody (Product # PA5-119907) at 1:1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa; Observed band size: 50 kDa (Glycoprotein). Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SH-SY5Y cells using IL2RG Polyclonal Antibody (Product # PA5-119907) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with IL2RG Polyclonal Antibody at 1:200 dilution in 2% BSA overnight at 4 8451. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SiHa cells using IL2RG Polyclonal Antibody (Product # PA5-119907) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with IL2RG Polyclonal Antibody at 1:200 dilution in 2% BSA overnight at 4°C. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human tonsil tissue with IL2RG Polyclonal Antibody (Product # PA5-119907) at a 1:600 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human spleen tissue with IL2RG Polyclonal Antibody (Product # PA5-119907) at a 1:600 dilution.