PA5-119907

antibody from Invitrogen Antibodies

Targeting: IL2RG CD132, CIDX, IMD4, SCIDX1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-119907

Provider

Invitrogen Antibodies

Product name

IL2RG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: Mouse spleen tissue lysates, SH-SY5Y, SiHa, human spleen tissue, human tonsil tissue.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IL-2RG on mouse spleen tissue withIL2RG Polyclonal Antibody (Product # PA5-119907) at 1:1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa; Observed band size: 50 kDa (Glycoprotein). Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SH-SY5Y cells using IL2RG Polyclonal Antibody (Product # PA5-119907) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with IL2RG Polyclonal Antibody at 1:200 dilution in 2% BSA overnight at 4 8451. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SiHa cells using IL2RG Polyclonal Antibody (Product # PA5-119907) at 1:200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.01% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with IL2RG Polyclonal Antibody at 1:200 dilution in 2% BSA overnight at 4°C. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human tonsil tissue with IL2RG Polyclonal Antibody (Product # PA5-119907) at a 1:600 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human spleen tissue with IL2RG Polyclonal Antibody (Product # PA5-119907) at a 1:600 dilution.