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- Antibody Data
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX100735 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100735, RRID:AB_1950490
- Product name
- HICE1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Regulation of microtubule-based microtubule nucleation by mammalian polo-like kinase 1.
Johmura Y, Soung NK, Park JE, Yu LR, Zhou M, Bang JK, Kim BY, Veenstra TD, Erikson RL, Lee KS
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 12;108(28):11446-51
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 12;108(28):11446-51
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB to detect cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image), using GTX100735 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SampleA: His-Hice1 (2x)B: His-Hice1 (1x)7.5% SDS PAGEGTX100735 diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of human osteosarcoma cell line U2OS, using HICE1(GTX100735) antibody at 1:50-1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (GTX100735) antibody at 1:50 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (GTX100735) antibody at 1:50 dilution.
