TA319224

antibody from OriGene

Targeting: HAUS8 HICE1, MGC20533, NY-SAR-48

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319224

Provider

OriGene

Product name

Rabbit polyclonal HICE1 PS70 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal HICE1 PS70 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

HAUS8

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.22 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 pS70 antibody at 0.5 ug/mL for overnight at 4°C. Secondary antibody: IRDye800„? Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot of Rabbit anti-HICE1 pS70 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 ?l per lane. Primary antibody: HICE1 pS70 antibody at 0.75 ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HICE1 pS70. Other band(s): HICE1 pS70 splice variants and isoforms.

Validation comment

WB