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LearnRP1056
antibody from Boster Biological Technology
Targeting: NCOA1
bHLHe74, F-SRC-1, KAT13A, NCoA-1, RIP160, SRC1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- RP1056 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-KAT13A/SRC1/NCOA1 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for SRC 1/NCOA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human. SRC 1/NCOA1 information: Molecular Weight: 156757 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Widely expressed.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of KAT13A/SRC1 using anti-KAT13A/SRC1 antibody (RP1056). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SK-OV-3 whole cell lysates, Lane 2: SGC-7901 whole cell lysates, Lane 3: Jurkat whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT13A/SRC1 antigen affinity purified polyclonal antibody (Catalog # RP1056) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT13A/SRC1 at approximately 157KD. The expected band size for KAT13A/SRC1 is at 157KD.
- Additional image
