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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-57160 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GKAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KNHQGKDRPL TVSLKDFHSE DHISKKTEEL SSSQTLSHDG GFFNRLEDDV HKILIREKRR EQLTEYNGTD NCTAHEHNQA LVLKDG
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GKAP1 in control (vector only transfected HEK293T lysate) and GKAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a GKAP1 Polyclonal Antibody (Product # PA5-57160).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GKAP1 in human cell line U-251 MG shows positivity in cytoplasm & centrosome. Samples were probed using a GKAP1 Polyclonal Antibody (Product # PA5-57160).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GKAP1 in human cell line U-2 OS using a GKAP1 Polyclonal Antibody (Product # PA5-57160) shows localization to cytosol and microtubule organizing center.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GKAP1 in human kidney using GKAP1 Polyclonal Antibody (Product # PA5-57160).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GKAP1 in human cerebral cortex using GKAP1 Polyclonal Antibody (Product # PA5-57160).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GKAP1 in human testis and pancreas tissues using GKAP1 Polyclonal Antibody (Product # PA5-57160). Corresponding GKAP1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GKAP1 in human cerebral cortex, kidney, pancreas and testis using GKAP1 Polyclonal Antibody (Product # PA5-57160) (A) shows similar protein distribution across tissues to an independent GKAP1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GKAP1 in human pancreas using GKAP1 Polyclonal Antibody (Product # PA5-57160) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GKAP1 in human testis using GKAP1 Polyclonal Antibody (Product # PA5-57160) shows high expression.
