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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30684 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDAH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, MCF-7.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDAH2 Polyclonal Antibody (Product # PA5-30684). Sample (30 µg of whole cell lysate). Lane A: MCF-7. 12% SDS PAGE. DDAH2 Polyclonal Antibody (Product # PA5-30684) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDAH2 Polyclonal Antibody detects DDAH2 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 15 min. Green: DDAH2 protein stained by DDAH2 Polyclonal Antibody (Product # PA5-30684) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDAH2 Polyclonal Antibody detects DDAH2 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. DDAH2 Polyclonal Antibody (Product # PA5-30684) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
