MAB2939

antibody from R&D Systems

Targeting: CA6

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

MAB2939

Provider

R&D Systems

Product name

Human Carbonic Anhydrase VI Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Carbonic Anhydrase 2, 3, 4, 5A, 5B, 7, 8, 9, recombinant mouse Carbonic Anhydrase 12, and 14 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

EAW71606


Isotype

IgG

Antibody clone number

401819

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Carbonic Anhydrase VI by Western Blot. Western blot shows human saliva. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Carbonic Anhydrase VI by Simple Western^TM. Simple Western lane view shows human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.