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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00000246-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000246-M04, RRID:AB_622268
- Product name
- ALOX15 monoclonal antibody (M04), clone 3D8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ALOX15.
- Antigen sequence
MGLYRIRVSTGASLYAGSNNQVQLWLVGQHGEAAL
GKRLWPARGKETELKVEVPEYLGPLLFVKLRKRHL
LKDDAWFCNWISVQGPGAGDEVRFPCYRWVEGNGV
LSLPEGTGRTVGEDPQGLFQKHREEELEERRKLYR
WGNWKDGLILNMAGAKLYDLPVDERFLEDKRVDFE
VSLAKGLADLAIKDSLNVLTCWKDLDDFNRIFWCG
QSKLAERVRDSWKEDALFGYQFLNGANPVVLRRSA
HLPARLVFPPGMEELQAQLEKELEGGTLFEADFSL
LDGIKANVILCSQQHLAAPLVMLKLQPDGKLLPMV
IQLQLPRTGSPPPPLFLPTDPPMAWLLAKCWVRSS
DFQLHELQSHLLRGHLMAEVIVVATMRCLPSIHPI
FKLIIPHLRYTLEINVRARTGLVSDMGIFDQIMST
GGGGHVQLLKQAGAFLTYSSFCPPDDLADRGLLGV
KSSFYPQDALRLWEIIYRYVEGIVSLHYKTDVAVK
DDPELQTWCREITEIGLQGAQDRGFPVSLQARDQV
CHFVTMCIFTCTGQHASVHLGQLDWYSWVPNAPCT
MRLPPPTTKDATLETVMATLPNFHQASLQMSITWQ
LGRRQPVMVAVGQHEEEYFSGPEPKAVLKKFREEL
AALDKEIEIRNAKLDMPYEYLRPSVVENSVAI- Isotype
- IgG
- Antibody clone number
- 3D8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia.
Differential expression and localization of 12/15 lipoxygenases in adipose tissue in human obese subjects.
Selective survival rescue in 15-lipoxygenase-1-deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1.
Gregus AM, Dumlao DS, Wei SC, Norris PC, Catella LC, Meyerstein FG, Buczynski MW, Steinauer JJ, Fitzsimmons BL, Yaksh TL, Dennis EA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 May;27(5):1939-49
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 May;27(5):1939-49
Differential expression and localization of 12/15 lipoxygenases in adipose tissue in human obese subjects.
Dobrian AD, Lieb DC, Ma Q, Lindsay JW, Cole BK, Ma K, Chakrabarti SK, Kuhn NS, Wohlgemuth SD, Fontana M, Nadler JL
Biochemical and biophysical research communications 2010 Dec 17;403(3-4):485-90
Biochemical and biophysical research communications 2010 Dec 17;403(3-4):485-90
Selective survival rescue in 15-lipoxygenase-1-deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1.
Calandria JM, Marcheselli VL, Mukherjee PK, Uddin J, Winkler JW, Petasis NA, Bazan NG
The Journal of biological chemistry 2009 Jun 26;284(26):17877-82
The Journal of biological chemistry 2009 Jun 26;284(26):17877-82
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ALOX15 monoclonal antibody (M04), clone 3D8 Western Blot analysis of ALOX15 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ALOX15 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ALOX15 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
