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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA504358 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504358, RRID:AB_11139708
- Product name
- ALOX15 mouse monoclonal antibody, clone OTI7H6 (formerly 7H6)
- Antibody type
- Monoclonal
- Description
- ALOX15 mouse monoclonal antibody, clone OTI7H6 (formerly 7H6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ALOX15
- Isotype
- IgG
- Antibody clone number
- OTI7H6
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Aging delays resolution of acute inflammation in mice: reprogramming the host response with novel nano-proresolving medicines.
Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits.
Self-limited versus delayed resolution of acute inflammation: temporal regulation of pro-resolving mediators and microRNA.
Arnardottir HH, Dalli J, Colas RA, Shinohara M, Serhan CN
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 15;193(8):4235-44
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 15;193(8):4235-44
Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits.
Chiang N, Shinohara M, Dalli J, Mirakaj V, Kibi M, Choi AM, Serhan CN
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6378-88
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6378-88
Self-limited versus delayed resolution of acute inflammation: temporal regulation of pro-resolving mediators and microRNA.
Fredman G, Li Y, Dalli J, Chiang N, Serhan CN
Scientific reports 2012;2:639
Scientific reports 2012;2:639
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX15 (RC206621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX15.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALOX15 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ALOX15 mouse monoclonal antibody (TA504358) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15(RC206621).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504358)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206621 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALOX15 antibody(TA504358), and then analyzed by flow cytometry.
- Validation comment
- FC
