16906-1-AP

antibody from Invitrogen Antibodies

Targeting: PIGT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16906-1-AP

Provider

Invitrogen Antibodies

Product name

PIGT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: RCTSISWEL RQTLSVVFDA FITGQGKKDW SLFRMFSRTL TEPCPLASES RVYVDITTYN QDNETLEVHP PPTTTYQDVI LGTRKTYAIY DLLDTAMINN SRNLNIQLKW KRPPENEAPP VPFLHAQRYV SGYGLQKGEL STLLYNTHPY RAFPVLLLDT VPWYLRLYVH TLTITSKGKE NKPSYIHYQP AQDRLQPHLL EMLIQLPANS VTKVSIQFER ALLKWTEYTP DPNHGFYVSP SVLSALVPSM VAAKPVDWEE SPLFNSLFPV SDGSNYFVRL YTEPLLVNLP TPDFSMPYNV ICLTCTVVAV CYGSFYNLLT RTFHIEEPRT GGLAKRLANL IRRARGVPPL (230-578 aa encoded by BC015022)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 16906-1-AP (PIGT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 16906-1-AP (PIGT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PIGT (IP:16906-1-AP, 4ug; Detection:16906-1-AP 1:300) with mouse liver tissue lysate 6000 ug.