Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 11363-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEDFARGAA SPGPSRPGLV PVSIIGAEDE DFENELETNS EEQNSQFQSL EQVKRRPAHL MALLQHVALQ FEPGPLVLRV PVPPNVAFEL DRTRADLISE DVQRRFVQEV VQSQQVAVGR QLEDFRSKRL MGMTPWEQEL AQLEAWVGRD RASYEARERH VAERLLMHLE EMQHTISTDE EKSAAVVNAI GLYMRHLGVR TKSGDKKSGR NFFRKKVMGN RRSDEPAKTK KGLSSILDAA RWNRGEPQVP DFRHLKAEVD AEKPGATDRK GGVGMPSRDR NIGAPGQDTP GVSLHPLSLD SPDREPGADA PLELGDSSPQ GPMSLESLAP PESTDEGAET ESPEPGDEGE P (1-350 aa encoded by BC011726)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.39 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ARHGEF1 (IP:11363-1-AP, 4ug; Detection:11363-1-AP 1:1000) with HEK-293 cells lysate 2800ug.