Explore
Validate
LearnPA5-31323
antibody from Invitrogen Antibodies
Targeting: GTPBP3
FLJ14700, GTPBG3, MSS1, MTGP1, THDF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-31323 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTPBP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG.
- Concentration
- 1 mg/mL
Submitted references A novel splice variant of Elp3/Kat9 regulates mitochondrial tRNA modification and function.
Boutoual R, Jo H, Heckenbach I, Tiwari R, Kasler H, Lerner CA, Shah S, Schilling B, Calvanese V, Rardin MJ, Scheibye-Knudsen M, Verdin E
Scientific reports 2022 Aug 31;12(1):14804
Scientific reports 2022 Aug 31;12(1):14804
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GTPBP3 Polyclonal Antibody (Product # PA5-31323). Sample (30 µg of whole cell lysate). Lane A: IMR32. 10% SDS PAGE. GTPBP3 Polyclonal Antibody (Product # PA5-31323) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GTPBP3 Polyclonal Antibody detects GTPBP3 protein at cytosol on human brain by immunohistochemical analysis. Sample: Paraffin-embedded human brain. GTPBP3 Polyclonal Antibody (Product # PA5-31323) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Overexpression of mt-ELP3 protects mt-tRNAs substrate from angiogenin-mediated cleavage without altering 2-thiolation levels. ( A ) and ( B ) Northern analysis of mt-tRNA Lys , mt-tRNA Leu , mt-tRNA Glu and mt-tRNA Val after in vitro angiogenin digestion of small RNAs purified from control cells and cells overexpressing mt-ELP3 ( A ), and from control cells and cells overexpressing mt-ELP3 mut ( B ) for the indicated times. The accompanying histogram shows the kinetic analysis of angiogenin digestions. The amounts of intact mt-tRNA after 0, 10, 20 and 30 min of incubation with angiogenin are represented as fold-changes relative to the undigested control (0 min). All data are mean +- SD of at least three experiments. * p < 0.05. ( C ) APM-northern analysis of 2-thiolation of mt-tRNA Lys , mt-tRNA Leu and mt-tRNA Glu from control cells and cells overexpressing mt-ELP3. The same amounts of total RNA (5 ug) were run in a denaturing polyacrylamide-urea gel in the presence (+) or absence (-) of APM. The thiolated tRNAs were detected as retarded bands in the presence of APM. The APM (-) membrane was probed with 5S rRNA as a loading control. ( D ) mt-ELP3 interacts with GTPBP3 . HEK 293T cells were co-transfected with pcDNA mt-ELP3-Flag and pCMV6 GTPBP3 plasmids as indicated, and the lysates were immunoprecipitated with anti-Flag antibody. Precipitated proteins were immunoblotted with anti-ELP3, GTPBP3 and VDAC antibodies. Beads mixed with lysate without anti-Flag antibody and beads
