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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB5560 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB5560, RRID:AB_10677200
- Product name
- SOS1 monoclonal antibody, clone AF3F11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant SOS1.
- Isotype
- IgG
- Antibody clone number
- AF3F11
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Structural basis of the differential binding of the SH3 domains of Grb2 adaptor to the guanine nucleotide exchange factor Sos1.
GEFs in growth factor signaling.
Identification of the mitogen-activated protein kinase phosphorylation sites on human Sos1 that regulate interaction with Grb2.
McDonald CB, Seldeen KL, Deegan BJ, Farooq A
Archives of biochemistry and biophysics 2008 Nov 1;479(1):52-62
Archives of biochemistry and biophysics 2008 Nov 1;479(1):52-62
GEFs in growth factor signaling.
Bernards A, Settleman J
Growth factors (Chur, Switzerland) 2007 Oct;25(5):355-61
Growth factors (Chur, Switzerland) 2007 Oct;25(5):355-61
Identification of the mitogen-activated protein kinase phosphorylation sites on human Sos1 that regulate interaction with Grb2.
Corbalan-Garcia S, Yang SS, Degenhardt KR, Bar-Sagi D
Molecular and cellular biology 1996 Oct;16(10):5674-82
Molecular and cellular biology 1996 Oct;16(10):5674-82
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Immunoblot analysis of cell lysates.Lane 1 : HeLa.Lane 2 : Molt 4.Lane 3 : HepG2.Lane 4 : NIH/3T3.Lane 5 : PC-12.
