PB9250

antibody from Boster Biological Technology

Targeting: CD63 ME491, MLA1, TSPAN30

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PB9250

Provider

Boster Biological Technology

Product name

Anti-CD63 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CD63 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CD63 information: Molecular Weight: 25637 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies; Tissue Specificity: Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Serum exosomes derived from spontaneously hypertensive rats induce cardiac hypertrophy in vitro and in vivo by increasing autocrine release of angiotensin II in cardiomyocytes.

Yu J, Tang Y, Wang Y, Zhou M, Li Y, Hong J, Li C, Xu B, Guo X, Mao J
Biochemical pharmacology 2023 Apr;210:115462

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Liposome fusogenic enzyme-free circuit enables high-fidelity determination of single exosomal RNA.

Sun Z, Chen X, Niu R, Chen H, Zhu Y, Zhang C, Wang L, Mou H, Zhang H, Luo Y
Materials today. Bio 2023 Apr;19:100613

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Tumor-Derived Exosomal miR-29b Reduces Angiogenesis in Pancreatic Cancer by Silencing ROBO1 and SRGAP2.

Wang L, Yang L, Zhuang T, Shi X
Journal of immunology research 2022;2022:4769385

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Cancer-Derived Small Extracellular Vesicles PICKER.

Chen X, Deng Y, Niu R, Sun Z, Batool A, Wang L, Zhang C, Ma N, Yang Q, Liu G, Yang J, Luo Y
Analytical chemistry 2022 Sep 27;94(38):13019-13027

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Hsp16.3 of mycobacterium tuberculosis in exosomes as a biomarker of tuberculosis.

Huang C, Pan L, Shen X, Tian H, Guo L, Zhang Z, Liu X
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 2021 Nov;40(11):2427-2430

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ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes.

Fang PP, Pan CW, Lin W, Li J, Huang SS, Zhou GY, Du WJ, Li Q
Mediators of inflammation 2020;2020:8183713

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Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle, Pelodiscus sinensis.

Zhu X, Wang S, Tarique I, An T, Yang H, Bai X, Wang X, Chen Q, Yang P
Frontiers in physiology 2019;10:1097

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Elevated Serum Exosomal miR-125b Level as a Potential Marker for Poor Prognosis in Intermediate-Risk Acute Myeloid Leukemia.

Jiang L, Deng T, Wang D, Xiao Y
Acta haematologica 2018;140(3):183-192

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Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A.

Du L, Li G, Yang Y, Yang G, Wan J, Ma Z, Hou Y
International journal of clinical and experimental pathology 2018;11(10):4879-4888

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Anti- CD63 Picoband antibody, PB9250, Western blottingAll lanes: Anti CD63 (PB9250) at 0.5ug/mlWB: Recombinant Human CD63 Protein 0.5ngPredicted bind size: 26KDObserved bind size: 26KD

Additional image