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Western blot
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Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
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- Product number
- LS-C682133 - Provider product page
- Provider
- LSBio
- Product name
- PGLS / 6PGL Antibody LS-C682133
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All Lanes:PGLS antibody at 4.19 ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Lane 3: U251 whole cell lysate Lane 4: Thp-1 whole cell lysate Lane 5: HepG-2 whole cell lysate Lane 6: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28 kDa Observed band size: 28,52 kDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All Lanes:PGLS antibody at 4.19 ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Lane 3: U251 whole cell lysate Lane 4: Thp-1 whole cell lysate Lane 5: HepG-2 whole cell lysate Lane 6: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28 kDa Observed band size: 28,52 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating PGLS in U251 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in U251 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ U251 whole cell lysate(500ug) Lane 3: U251 whole cell lysate (20ug)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating PGLS in U251 whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in U251 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ U251 whole cell lysate(500ug) Lane 3: U251 whole cell lysate (20ug)
