PA5-31678

antibody from Invitrogen Antibodies

Targeting: PGLS 6PGL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31678

Provider

Invitrogen Antibodies

Product name

PGLS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH. Predicted reactivity: Mouse (88%), Rat (88%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PGLS Polyclonal Antibody (Product # PA5-31678). Sample (30 µg of whole cell lysate). Lane A: SK-N-SH. 12% SDS PAGE. PGLS Polyclonal Antibody (Product # PA5-31678) diluted at 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PGLS Polyclonal Antibody (Product # PA5-31678). Sample (30 µg of whole cell lysate). Lane A: SK-N-SH. 12% SDS PAGE. PGLS Polyclonal Antibody (Product # PA5-31678) diluted at 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PGLS showing staining in the cytoplasm of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PGLS polyclonal antibody (Product # PA5-31678) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PGLS was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PGLS Polyclonal Antibody (Product # PA5-31678) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PGLS (Product # PA5-31678) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PGLS Polyclonal Antibody detects PGLS protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. PGLS Polyclonal Antibody (Product # PA5-31678) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.