PA5-96463

antibody from Invitrogen Antibodies

Targeting: RPS3A MFTL, S3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96463

Provider

Invitrogen Antibodies

Product name

RPS3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITED VQGKNCLTNF HGMDLTRDKM CSMVKKWQTM IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND LKEVVNKLIP DSIGKDIEKA CQSIYPLHDV FVRKVKMLKK PKFELGKLME LHGEGSSSGK ATGDETGAKV ERADGYEPPV QESV; Positive Samples: MCF7, 293T, A-549, Mouse liver, Mouse lung, Mouse ovary, Mouse pancreas, Rat ovary; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.84 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPS3A in extracts of various cell lines using RPS3A Polyclonal Antibody (Product # PA5-96463) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPS3A was performed in MCF-7 cells using RPS3A Polyclonal Antibody (Product # PA5-96463).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS3A in paraffin-embedded rat brain using RPS3A Polyclonal Antibody (Product # PA5-96463) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS3A in paraffin-embedded human liver cancer using RPS3A Polyclonal Antibody (Product # PA5-96463) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPS3A in paraffin-embedded mouse brain using RPS3A Polyclonal Antibody (Product # PA5-96463) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of RPS3A was performed in 200 µg extracts of MCF7 cells using RPS3A Polyclonal Antibody (Product # PA5-96463). Western blot was performed from the immunoprecipitate using RPS3A Polyclonal Antibody at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of RPS3A was performed in 200 µg extracts of MCF7 cells using RPS3A Polyclonal Antibody (Product # PA5-96463). Western blot was performed from the immunoprecipitate using RPS3A Polyclonal Antibody at a dilution of 1:500.